Anderson C F, Record M T
Department of Chemistry, University of Wisconsin-Madison 53706, USA.
Annu Rev Phys Chem. 1995;46:657-700. doi: 10.1146/annurev.pc.46.100195.003301.
Coulombic interactions of salt ions with polymeric and oligomeric nucleic acids in solution have large and distinctive effects on ion distributions, on thermodynamic coefficients, and hence on equilibrium processes involving nucleic acids, such as their conformational transitions and binding interactions. In experimental or theoretical studies where an oligonucleotide is taken to represent the corresponding polynucleotide, the impact of coulombic end effects on molecular and thermodynamic properties must be taken into account. Observable consequences of coulombic interactions in nucleic acid solutions have been calculated by using models with varying degrees of detail and methods formulated at varying levels of rigor. From comparisons of experimental results with predictions of the prevalent theoretical approaches, this review concludes that the more rigorous methods have proved capable of accounting for thermodynamic (and some molecular) consequences of coulombic interactions with a minimal number of preaveraged parameters that represent the most important structural features of the nucleic acid solution.
溶液中盐离子与聚合和寡聚核酸的库仑相互作用对离子分布、热力学系数有很大且独特的影响，因此对涉及核酸的平衡过程也有影响，比如它们的构象转变和结合相互作用。在将寡核苷酸视为相应多核苷酸的实验或理论研究中，必须考虑库仑末端效应对分子和热力学性质的影响。通过使用具有不同详细程度的模型和不同严格程度制定的方法，已计算出核酸溶液中库仑相互作用的可观察结果。通过将实验结果与流行理论方法的预测进行比较，本综述得出结论，更严格的方法已证明能够用最少数量的预平均参数来解释库仑相互作用的热力学（以及一些分子）结果，这些参数代表了核酸溶液最重要的结构特征。
