Gamzatova Zaynab, Komlichenko Eduard, Kostareva Anna, Galagudza Mikhail, Ulrikh Elena, Zubareva Tatiana, Sheveleva Tatiana, Nezhentseva Elena, Kalinina Evgenia
Federal Almazov Medical Research Centre , Saint Petersburg , Russia .
Gynecol Endocrinol. 2014 Oct;30 Suppl 1:43-7. doi: 10.3109/09513590.2014.945789.
To review the literature and to present the latest advances in the autotransplantation of cryopreserved ovarian tissue.
A literature review was conducted for all relevant articles assessing the fertility preservation, ovarian tissue transplantation, standard freezing and vitrification of ovarian tissue.
One of the promising and effective methods for fertility preservation may be the autotransplantation of cryopreserved ovarian tissue. At present, 30 babies have been born after orthotopic autotransplantation of frozen-thawed human ovarian tissue. Restoration of ovarian activity occurs between 3.5 months and 6.5 months. The longevity of autotransplanted ovarian tissue is about 5-7 years. The follicles are similarly preserved after all freezing methods; however, the ovarian stroma is significantly better preserved after vitrification than after slow freezing. An important topic for further research is preparation of the "vascular bed", optimization of vitrification technique and the development of alternative procedures to avoid the transmission of cancer cells via ovarian tissue autotransplantation - "artificial ovary".
Cryopreservation of ovarian tissue has unique advantages over other strategies. This method: (1) does not delay cancer treatment; (2) is safer for hormone dependent malignancy; (3) can be done independent of menstrual cycles; (4) is the only option for prepubertal girls; (5) can restore not only fertility but endocrine function.
回顾文献并介绍冷冻保存卵巢组织自体移植的最新进展。
对所有评估生育力保存、卵巢组织移植、卵巢组织标准冷冻和玻璃化的相关文章进行文献综述。
冷冻保存卵巢组织自体移植可能是一种有前景且有效的生育力保存方法。目前，冻融后的人卵巢组织原位自体移植后已有30名婴儿出生。卵巢活动在3.5个月至6.5个月之间恢复。自体移植卵巢组织的寿命约为5至7年。所有冷冻方法后卵泡的保存情况相似；然而，玻璃化后卵巢基质的保存明显优于慢速冷冻。进一步研究的一个重要课题是“血管床”的制备、玻璃化技术的优化以及开发替代程序以避免癌细胞通过卵巢组织自体移植（“人工卵巢”）传播。
卵巢组织冷冻保存相对于其他策略具有独特优势。该方法：（1）不延迟癌症治疗；（2）对激素依赖性恶性肿瘤更安全；（3）可独立于月经周期进行；（4）是青春期前女孩的唯一选择；（5）不仅能恢复生育能力，还能恢复内分泌功能。
