Jilin Ginseng Academy, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
School of Pharmaceutical, Changchun University of Chinese Medicine, Jilin, Changchun 130117, China.
Anal Chim Acta. 2014 Oct 3;845:70-6. doi: 10.1016/j.aca.2014.06.014. Epub 2014 Jun 12.
Direct analysis in real time-mass spectrometry (DART-MS) was employed as a novel fast method to identify traditional Chinese herbal medicine (TCHM). In order to obtain high quality mass spectra, the ionization temperature was optimized for every kind of sample. With minimal or no sample pretreatment, major TCHM components, including alkaloids, flavonoids and some ginsenosides, were directly detected within several seconds, while thirteen ginsenosides need derivatization to get good mass spectra. Pseudoginsenoside F11, compound K, protopanaxatriol (PPT) and protopanaxadiol (PPD), for the first time were detected without derivatization. Among five of eight tested Chinese herbal medicines, Rhizoma Corydalis, Bulbus Fritillariae Thunbergii, Arecae Semen, Ramulus Uncariae Cum Uncis and Scutellariae Radix, were first time identified by DART-MS. In addition, the ionization mechanisms of major herbal components, alkaloids, flavonoids and ginsenosides, were discussed in detail. Our results demonstrated that DART-MS could provide a rapid, reliable and environmental friendly method for the rapid identification of TCHM, and may be applicable to other plants.
直接分析实时质谱法（DART-MS）被用作一种新颖的快速方法来鉴定中药（TCM）。为了获得高质量的质谱，针对每种样品优化了离子化温度。在不需要或只需极少的样品预处理的情况下，主要的 TCM 成分，包括生物碱、黄酮类和一些人参皂苷，可在几秒钟内直接检测到，而 13 个人参皂苷需要衍生化才能得到良好的质谱。伪原人参二醇 F11、化合物 K、原人参三醇（PPT）和原人参二醇（PPD）首次无需衍生化即可检测到。在 8 种中草药中的 5 种中，延胡索、川贝母、槟榔、钩藤和黄芩首次通过 DART-MS 鉴定。此外，还详细讨论了主要草药成分、生物碱、黄酮类和人参皂苷的离子化机制。我们的结果表明，DART-MS 可为 TCM 的快速鉴定提供一种快速、可靠和环保的方法，并且可能适用于其他植物。
