Liu Chengyuan, Wen Wu, Shao Jun, Zhao Wan, Qi Keke, Yang Jiuzhong, Pan Yang
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230029, P.R. China.
The First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei, Anhui, 230031, P.R. China.
Rapid Commun Mass Spectrom. 2017 Sep 30;31(18):1491-1498. doi: 10.1002/rcm.7929.
The goal of this work is to employ extractive atmospheric pressure photoionization mass spectrometry (EAPPI-MS) to characterize the constituents in traditional Chinese herbal medicine (TCHM) directly without chromatographic separation.
Sample was placed in 4 mL of methanol/water (v/v, 3:1) in the nebulization cell, and then the ultrasonic nebulizer was switched on. The ultrasonic nebulization system allows the simultaneous sample extraction and introduction of extract aerosols. The extract aerosols were vaporized in a transfer tube. Mixed with a gaseous dopant, vaporized analytes were ionized through ambient photon-induced ion-molecule reactions, and were mass-analyzed by high-resolution time-of-flight mass spectrometry (TOF-MS).
The major ingredients including alkaloids, flavonoids, amino acids, saccarides, ginsenosides, lignans and terpenoids were readily detected. Compared with electrospray ionization (ESI), EAPPI allowed the ionization of a wider range of compounds, which is desirable for the integral characterization of TCHMs containing numerous constituents. The significant discrepancies for both alkaloids and terpenoids in tripterygium glycoside tablets from two different manufacturers could be simultaneously reflected from EAPPI mass spectra.
Our results demonstrate that EAPPI-MS can be regarded as a supplementary ambient method for the fast and comprehensive analysis of TCHMs, which is important for the quality control and safety assurance of these products.
这项工作的目标是采用萃取常压光电离质谱法（EAPPI-MS）直接对中药成分进行表征，无需色谱分离。
将样品置于雾化池中4 mL甲醇/水（体积比3:1）中，然后开启超声雾化器。超声雾化系统可同时进行样品萃取和萃取气溶胶的引入。萃取气溶胶在传输管中汽化。与气态掺杂剂混合后，汽化的分析物通过环境光子诱导的离子-分子反应进行电离，并通过高分辨率飞行时间质谱（TOF-MS）进行质量分析。
容易检测到主要成分，包括生物碱、黄酮类化合物、氨基酸、糖类、人参皂苷、木脂素和萜类化合物。与电喷雾电离（ESI）相比，EAPPI能使更广泛的化合物电离，这对于含有众多成分的中药整体表征是很理想的。来自两个不同制造商的雷公藤多苷片中生物碱和萜类化合物的显著差异可同时从EAPPI质谱图中反映出来。
我们的结果表明，EAPPI-MS可被视为一种用于快速全面分析中药的补充常压方法，这对这些产品的质量控制和安全保证很重要。
