El-Sayed El-Sayed M, Mansour Ahmed M, Nady Mohamed E
Pharmacology and Toxicology Department, Faculty of Pharmacy, Al-Azhar University, Nasr-City, Cairo, Egypt.
J Biochem Mol Toxicol. 2015 Jan;29(1):35-42. doi: 10.1002/jbt.21604. Epub 2014 Sep 9.
The present study was undertaken to evaluate the protective effect of pterostilbene against acetaminophen-induced hepatotoxicity. Silymarin was used as a standard hepatoprotective agent. A single dose of acetaminophen (800 mg/kg i.p.), injected to male rats, caused significant increases in serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin, total cholesterol, triglycerides, tumor necrosis factor alpha, and hepatic contents of malondialdehyde, nitric oxide, caspase-3, hydroxyproline, with significant decreases in serum HDL-cholesterol, total proteins, albumin, and hepatic activities of reduced glutathione, superoxide dismutase and catalase as compared with the control group. On the other hand, administration of each of pterostilbene (50 mg/kg, p.o.) and silymarin (100 mg/kg, p.o.) for 15 days before acetaminophen ameliorated liver function and oxidative stress parameters. Histopathological evidence confirmed the protection offered by pterostilbene from the tissue damage caused by acetaminophen. In conclusion, pterostilbene possesses multimechanistic hepatoprotective activity that can be attributed to its antioxidant, anti-inflammatory, and antiapoptotic actions.
本研究旨在评估紫檀芪对乙酰氨基酚诱导的肝毒性的保护作用。水飞蓟宾用作标准肝保护剂。向雄性大鼠腹腔注射单剂量的乙酰氨基酚（800 mg/kg），导致血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、胆红素、总胆固醇、甘油三酯、肿瘤坏死因子α以及肝脏丙二醛、一氧化氮、半胱天冬酶-3、羟脯氨酸含量显著升高，与对照组相比，血清高密度脂蛋白胆固醇、总蛋白、白蛋白以及肝脏还原型谷胱甘肽、超氧化物歧化酶和过氧化氢酶活性显著降低。另一方面，在给予乙酰氨基酚前15天，分别口服紫檀芪（50 mg/kg）和水飞蓟宾（100 mg/kg）可改善肝功能和氧化应激参数。组织病理学证据证实了紫檀芪对乙酰氨基酚所致组织损伤的保护作用。总之，紫檀芪具有多机制的肝保护活性，这可归因于其抗氧化、抗炎和抗凋亡作用。
