Mondal Bama Charan, Shim Jiwon, Evans Cory J, Banerjee Utpal
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States.
Elife. 2014 Sep 8;3:e03626. doi: 10.7554/eLife.03626.
Blood progenitors within the lymph gland, a larval organ that supports hematopoiesis in Drosophila melanogaster, are maintained by integrating signals emanating from niche-like cells and those from differentiating blood cells. We term the signal from differentiating cells the 'equilibrium signal' in order to distinguish it from the 'niche signal'. Earlier we showed that equilibrium signaling utilizes Pvr (the Drosophila PDGF/VEGF receptor), STAT92E, and adenosine deaminase-related growth factor A (ADGF-A) (Mondal et al., 2011). Little is known about how this signal initiates during hematopoietic development. To identify new genes involved in lymph gland blood progenitor maintenance, particularly those involved in equilibrium signaling, we performed a genetic screen that identified bip1 (bric à brac interacting protein 1) and Nucleoporin 98 (Nup98) as additional regulators of the equilibrium signal. We show that the products of these genes along with the Bip1-interacting protein RpS8 (Ribosomal protein S8) are required for the proper expression of Pvr.
在果蝇中，淋巴腺是支持造血作用的幼虫器官，其中的血液祖细胞通过整合来自类龛细胞和分化血细胞的信号得以维持。为了将其与“龛信号”区分开来，我们将来自分化细胞的信号称为“平衡信号”。此前我们发现，平衡信号传导利用了Pvr（果蝇血小板衍生生长因子/血管内皮生长因子受体）、STAT92E和腺苷脱氨酶相关生长因子A（ADGF-A）（蒙达尔等人，2011年）。关于该信号在造血发育过程中如何启动，我们知之甚少。为了鉴定参与淋巴腺血液祖细胞维持的新基因，特别是那些参与平衡信号传导的基因，我们进行了一项基因筛选，确定了bip1（bric à brac相互作用蛋白1）和核孔蛋白98（Nup98）是平衡信号的额外调节因子。我们发现，这些基因的产物与Bip1相互作用蛋白RpS8（核糖体蛋白S8）对于Pvr的正确表达是必需的。
