Quilliam M A, Thomson B A, Scott G J, Siu K W
Atlantic Research Laboratory, National Research Council of Canada, Halifax, N.S.
Rapid Commun Mass Spectrom. 1989 May;3(5):145-50. doi: 10.1002/rcm.1290030508.
Ion-spray mass spectrometry was investigated for the analysis of three marine neurotoxins: domoic acid, saxitoxin and tetrodotoxin. All three compounds gave positive-ion spectra with abundant ions of protonated molecules and no significant fragmentation. Domoic acid gave a negative-ion spectrum with a strong [M-H]- ion. Tandem mass spectrometry provided useful fragment-ion spectra for all compounds. Detection limits for flow injection analyses with selected-ion monitoring were determined to be 30 pg for saxitoxin, 100 pg for domoic acid and 200 pg for tetrodotoxin. Combining liquid chromatography with ion-spray mass spectrometry allowed the determination of domoic acid and some of its isomers in toxic shellfish tissue extracts.
软骨藻酸、石房蛤毒素和河豚毒素。这三种化合物均给出了质子化分子离子丰富且无明显碎片的正离子谱。软骨藻酸给出了具有强[M-H]-离子的负离子谱。串联质谱法为所有化合物提供了有用的碎片离子谱。采用选择离子监测的流动注射分析的检测限确定为：石房蛤毒素30皮克、软骨藻酸100皮克、河豚毒素200皮克。液相色谱与离子喷雾质谱联用能够测定有毒贝类组织提取物中的软骨藻酸及其一些异构体。
