Wang P P, Ren M, Chen S Q, Yin S W, Zhao C, Zhang H Y, Li X R, Cao Q Q, Zhou G Q
College of Life Sciences, Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, , , China.
, , China.
Genet Mol Res. 2017 May 10;16(2):gmr-16-02-gmr.16029129. doi: 10.4238/gmr16029129.
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two individuals from a wild population area (Jiande, Zhejiang Province, China). The number of alleles per locus varied from two to eight, the observed heterozygosity (H) ranged from 0.03571 to 0.9375, and the expected heterozygosity (H) ranged from 0.14326 to 0.81549. The average number of alleles, H, and H were 5.0, 0.4467, and 0.5518, respectively. By the calculation, the range of polymorphism information content (PIC) was 0.1177-0.8492. Most of the loci showed moderate or high polymorphism. These newly developed EST-simple sequence repeat (EST-SSR) markers would serve as an efficient tool for analyzing population connectivity and provide sufficient information for genetic diversity research, parentage, and molecular breeding of O. potamophila and other fishes with similar genetic relationship.
表达序列标签（ESTs）是微卫星开发的来源。在本研究中，我们通过使用Illumina RNA测序分离并鉴定了河川沙塘鳢的微卫星标记。我们对大量ESTs进行了测序，并筛选出200个潜在的微卫星。结果，在来自野生种群区域（中国浙江省建德）的32个个体中总共鉴定出56个新的多态性微卫星重复标记。每个位点的等位基因数量从2到8不等，观察到的杂合度（H）范围为0.03571至0.9375，预期杂合度（H）范围为0.14326至0.81549。等位基因的平均数量、H和H分别为5.0、0.4467和0.5518。经计算，多态性信息含量（PIC）范围为0.1177 - 0.8492。大多数位点显示出中等或高度多态性。这些新开发的EST - 简单序列重复（EST - SSR）标记将作为分析种群连通性的有效工具，并为河川沙塘鳢及其他具有相似遗传关系的鱼类的遗传多样性研究、亲权鉴定和分子育种提供充分的信息。
