分离并鉴定 27 个微卫星标记，用于特有种多花滇石栎（杜鹃花科）。
Isolation and characterization of 27 microsatellite markers for the endemic species Diplarche multiflora (Ericaceae).
机构信息
Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China ; Graduate School of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China.
出版信息
Appl Plant Sci. 2013 Jan 2;1(1). doi: 10.3732/apps.1200235. eCollection 2013 Jan.
PREMISE OF THE STUDY
Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. •
METHODS AND RESULTS
Twenty-seven microsatellite loci were isolated from the genome of D. multiflora using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Of these markers, 17 were polymorphic, and the number of alleles for the polymorphic microsatellite markers ranged from two to four, with an average of 2.2 per allele. The observed and expected heterozygosities varied from 0.0000 to 1.0000 and from 0.0000 to 0.7826, respectively. •
CONCLUSIONS
These polymorphic microsatellite markers will be useful for population genetic studies and for assessing the genetic diversity of this alpine species.
研究前提
从多花鹅绒藤的基因组中开发和特征化微卫星标记，以研究其遗传多样性和种群结构。
方法和结果
使用快速 AFLP 序列中重复序列分离（FIASCO）协议，从多花鹅绒藤的基因组中分离出 27 个微卫星标记。这些标记中，17 个为多态性，多态性微卫星标记的等位基因数范围从两个到四个，每个等位基因的平均值为 2.2。观察到的和预期的杂合度分别从 0.0000 到 1.0000 和从 0.0000 到 0.7826 变化。
结论
这些多态性微卫星标记将有助于群体遗传研究和评估这种高山物种的遗传多样性。
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