开发并鉴定九微卫星位点用于濒危树种华山松（松科）。
Development and characterization of nine microsatellites for an endangered tree, Pinus wangii (Pinaceae).
机构信息
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
出版信息
Appl Plant Sci. 2013 Jan 31;1(2). doi: 10.3732/apps.1200134. eCollection 2013 Feb.
PREMISE OF THE STUDY
Pinus wangii is an endemic and endangered species in southwestern China, and microsatellite primers were developed to characterize its genetic diversity and population structure. •
METHODS AND RESULTS
Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, nine sets of microsatellite primers were developed in P. wangii. One population with 26 individuals of P. wangii, as well as 11 individuals each for two congeneric species, P. taiwanensis and P. squamata, were used to test their polymorphism and transferability. The number of alleles per locus ranged from one to seven with an average of 3.7, and the observed heterozygosity and expected heterozygosity ranged from 0 to 0.91 and 0 to 0.75, respectively. •
CONCLUSIONS
We developed nine sets of polymorphic microsatellite loci that are suitable for investigating genetic diversity and population structure of P. wangii, and these markers may be useful for other Pinus species.
研究前提
松潘冷杉是中国西南地区特有的濒危物种，本研究开发了微卫星引物来对其遗传多样性和种群结构进行分析。
方法和结果
本研究利用序列中富含扩增片段的简单重复（FIASCO）技术开发了 9 对松潘冷杉微卫星引物。利用这些引物对 1 个 26 个个体的种群，以及 2 个同种的 11 个个体（包括台湾杉和翠柏）进行了多态性和可转移性检验。每个位点的等位基因数范围从 1 到 7，平均值为 3.7，观测杂合度和期望杂合度范围分别为 0 到 0.91 和 0 到 0.75。
结论
本研究开发了 9 对多态性微卫星标记，适合用于研究松潘冷杉的遗传多样性和种群结构，这些标记可能对其他松属物种也有帮助。
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