日本筋骨草和细梗筋骨草（唇形科）微卫星标记的开发。
Development of microsatellite markers for Leucosceptrum japonicum and L. stellipilum (Lamiaceae).
机构信息
Botanical Gardens, Tohoku University, Aoba, Sendai 980-0862, Japan.
出版信息
Appl Plant Sci. 2013 Oct 1;1(10). doi: 10.3732/apps.1300038. eCollection 2013 Oct.
PREMISE OF THE STUDY
We developed 10 new primers for amplifying microsatellite loci for both Leucosceptrum japonicum and L. stellipilum to study the genetic structure of hybrid zones between these two species. •
METHODS AND RESULTS
We isolated DNA fragments containing microsatellites from an enriched library and designed the 10 primer pairs amplifying the fragments. The number of alleles per locus ranged from one to 11 in both L. japonicum and L. stellipilum. The observed heterozygosity ranged from 0.00 to 0.84 and 0.00 to 1.00 in L. japonicum and L. stellipilum, respectively. The expected heterozygosity ranged from 0.00 to 0.84 and 0.00 to 0.89 in L. japonicum and L. stellipilum, respectively. •
CONCLUSIONS
These newly developed markers will be useful in investigating genetic structure and hybridization patterns in hybrid zones between L. japonicum and L. stellipilum.
研究前提
我们开发了 10 对新的引物，用于扩增 Leucosceptrum japonicum 和 L. stellipilum 的微卫星位点，以研究这两个物种之间杂交带的遗传结构。
方法和结果
我们从富集文库中分离出含有微卫星的 DNA 片段，并设计了 10 对扩增这些片段的引物。每个位点的等位基因数在 L. japonicum 和 L. stellipilum 中均为 1 至 11 个。在 L. japonicum 和 L. stellipilum 中，观察到的杂合度分别为 0.00 至 0.84 和 0.00 至 1.00。预期杂合度分别为 0.00 至 0.84 和 0.00 至 0.89。
结论
这些新开发的标记物将有助于研究 L. japonicum 和 L. stellipilum 之间杂交带的遗传结构和杂交模式。
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