开发用于半天然草原草本植物日本腹水草（车前科）的微卫星标记。
Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae).
作者信息
Nakahama Naoyuki, Izuno Ayako, Arima Kurumi, Isagi Yuji
机构信息
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
出版信息
Appl Plant Sci. 2016 Feb 11;4(2). doi: 10.3732/apps.1500096. eCollection 2016 Feb.
PREMISE OF THE STUDY
Veronicastrum japonicum (Plantaginaceae) grows in grasslands on Honshu Island, Japan, and is threatened by habitat loss because of rapid land development over recent decades. For the genetic characterization of the remaining populations, microsatellite markers were developed.
METHODS AND RESULTS
Twelve polymorphic microsatellite loci were developed using next-generation sequencing. The number of alleles per locus ranged from two to 24 (mean 7.7), and the expected heterozygosity per locus ranged from 0.35 to 0.94 (mean 0.68).
CONCLUSIONS
These markers can be used for genetic studies in conservation, such as the evaluation of genetic diversity and genetic structure.
研究前提
日本腹水草（车前科）生长于日本本州岛的草原上，近几十年来由于土地的快速开发，其栖息地丧失，面临生存威胁。为了对剩余种群进行遗传特征分析，开发了微卫星标记。
方法与结果
利用二代测序技术开发了12个多态性微卫星位点。每个位点的等位基因数从2个到24个不等（平均为7.7个），每个位点的期望杂合度从0.35到0.94不等（平均为0.68）。
结论
这些标记可用于保护遗传学研究，如遗传多样性和遗传结构评估。
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