Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada.
Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada ; Department of Plant Pathology, University of California, Davis Davis, CA, USA.
Front Plant Sci. 2014 Dec 5;5:676. doi: 10.3389/fpls.2014.00676. eCollection 2014.
Lentil (Lens culinaris Medik.) is a global food crop with increasing importance for food security in south Asia and other regions. Lens ervoides, a wild relative of cultivated lentil, is an important source of agronomic trait variation. Lens is a member of the galegoid clade of the Papilionoideae family, which includes other important dietary legumes such as chickpea (Cicer arietinum) and pea (Pisum sativum), and the sequenced model legume Medicago truncatula. Understanding the genetic structure of Lens spp. in relation to more fully sequenced legumes would allow leveraging of genomic resources. A set of 1107 TOG-based amplicons were identified in L. ervoides and a subset thereof used to design SNP markers for mapping. A map of L. ervoides consisting of 377 SNP markers spread across seven linkage groups was developed using a GoldenGate genotyping array and single SNP marker assays. Comparison with maps of M. truncatula and L. culinaris documented considerable shared synteny and led to the identification of a few major translocations and a major inversion that distinguish Lens from M. truncatula, as well as a translocation that distinguishes L. culinaris from L. ervoides. The identification of chromosome-level differences among Lens spp. will aid in the understanding of introgression of genes from L. ervoides into cultivated L. culinaris, furthering genetic research and breeding applications in lentil.
兵豆（Lens culinaris Medik.）是一种全球性的粮食作物，对南亚和其他地区的粮食安全越来越重要。栽培兵豆的野生近缘种兵豆（Lens ervoides）是农艺性状变异的重要来源。兵豆属于菜豆族Galegoid 分支，该分支包括其他重要的食用豆类，如鹰嘴豆（Cicer arietinum）和豌豆（Pisum sativum），以及已测序的模式豆科植物蒺藜苜蓿（Medicago truncatula）。了解 Lens spp.与更充分测序的豆科植物的遗传结构将允许利用基因组资源。在 L. ervoides 中鉴定出了一套 1107 个基于 TOG 的扩增子，并从中选择了一些 SNP 标记用于作图。使用 GoldenGate 基因分型阵列和单 SNP 标记分析，开发了由 377 个 SNP 标记组成的 L. ervoides 图谱，这些标记分布在 7 个连锁群中。与蒺藜苜蓿和兵豆的图谱进行比较，记录了相当大的共线性，并确定了几个主要的易位和一个主要的倒位，这些易位和倒位将 Lens 与蒺藜苜蓿区分开来，同时也将 L. culinaris 与 L. ervoides 区分开来。Lens spp. 之间染色体水平差异的鉴定将有助于理解从 L. ervoides 向栽培的 L. culinaris 导入基因，进一步推动了兵豆的遗传研究和育种应用。
