Wang Qi, Liu Yuqiang, He Jun, Zheng Xiaoming, Hu Jinlong, Liu Yanling, Dai Huimin, Zhang Yingxin, Wang Baoxiang, Wu Weixun, Gao He, Zhang Yunhui, Tao Xiaorong, Deng Huafeng, Yuan Dingyang, Jiang Ling, Zhang Xin, Guo Xiuping, Cheng Xianian, Wu Chuanyin, Wang Haiyang, Yuan Longping, Wan Jianmin
1] National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China [2].
National key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China.
Nat Commun. 2014 Sep 9;5:4768. doi: 10.1038/ncomms5768.
Rice stripe virus (RSV) causes one of the most serious viral diseases of rice (Oryza sativa L.), but the molecular basis of RSV resistance has remained elusive. Here we show that the resistant allele of rice STV11 (STV11-R) encodes a sulfotransferase (OsSOT1) catalysing the conversion of salicylic acid (SA) into sulphonated SA (SSA), whereas the gene product encoded by the susceptible allele STV11-S loses this activity. Sequence analyses suggest that the STV11-R and STV11-S alleles were predifferentiated in different geographic populations of wild rice, Oryza rufipogon, and remained prevalent in cultivated indica and japonica rice varieties, respectively. Introgression of the STV11-R allele into susceptible cultivars or heterologous transfer of STV11-R into tobacco plants confers effective resistance against RSV. Our results shed new insights into plant viral defense mechanisms and suggest effective means of breeding RSV-resistant crops using molecular marker-assisted selection or genetic engineering.
水稻条纹病毒（RSV）引发水稻（Oryza sativa L.）最严重的病毒病之一，但RSV抗性的分子基础一直难以捉摸。在此，我们表明水稻STV11的抗性等位基因（STV11-R）编码一种磺基转移酶（OsSOT1），催化水杨酸（SA）转化为磺化SA（SSA），而感病等位基因STV11-S编码的基因产物则失去了这种活性。序列分析表明，STV11-R和STV11-S等位基因在野生稻（Oryza rufipogon）的不同地理种群中预先分化，并分别在栽培籼稻和粳稻品种中普遍存在。将STV11-R等位基因导入感病品种或把STV11-R异源转移到烟草植株中，可赋予对RSV的有效抗性。我们的结果为植物病毒防御机制提供了新见解，并提出了利用分子标记辅助选择或基因工程培育抗RSV作物的有效方法。
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