Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, 250004, India.
Council of Scientific & Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, India.
Mol Biol Rep. 2024 Sep 4;51(1):956. doi: 10.1007/s11033-024-09899-3.
Sulphotransferase (SOT) enzyme (encoded by a conserved family of SOT genes) is involved in sulphonation of a variety of compounds, through transfer of a sulphuryl moiety from 3'phosphoadenosine- 5'phosphosulphate (PAPS) to a variety of secondary metabolites. The PAPS itself is derived from 3'adenosine-5'phosphosulphate (APS) that is formed after uptake of sulphate ions from the soil. The process provides tolerance against abiotic stresses like drought and heat in plants. Therefore, a knowledge of SOT genes in any crop may help in designing molecular breeding methods for improvement of tolerance for drought and heat.
Sequences of rice SOT genes and SOT domain (PF00685) of corresponding proteins were both used for identification of SOT genes in wheat and six related species (T. urartu, Ae. tauschii, T. turgidum, Z. mays, B. distachyon and Hordeum vulgare), although detailed analysis was conducted only in wheat. The wheat genes were mapped on individual chromosomes and also subjected to synteny and collinearity analysis. The proteins encoded by these genes were examined for the presence of a complete SOT domain using 'Conserved Domain Database' (CDD) search tool at NCBI.
In wheat, 107 TaSOT genes, ranging in length from 969 bp to 7636 bp, were identified and mapped onto individual chromosomes. SSRs (simple sequence repeats), microRNAs, long non-coding RNAs (lncRNAs) and their target sites were also identified in wheat SOT genes. SOT proteins were also studied in detail. An expression assay of TaSOT genes via wheat RNA-seq data suggested engagement of these genes in growth, development and responses to various hormones and biotic/abiotic stresses.
The results of the present study should help in further functional characterization of SOT genes in wheat and other related crops.
磺基转移酶（SOT）酶（由 SOT 基因家族的保守基因编码）参与各种化合物的磺化作用，通过将硫酰基部分从 3'腺嘌呤-5'磷酸硫酸（PAPS）转移到各种次级代谢物中。PAPS 本身来源于 3'腺苷-5'磷酸硫酸（APS），APS 是在从土壤中吸收硫酸盐离子后形成的。该过程为植物提供了对干旱和高温等非生物胁迫的耐受性。因此，任何作物的 SOT 基因知识都有助于设计分子育种方法，以提高对干旱和高温的耐受性。
使用水稻 SOT 基因序列和相应蛋白质的 SOT 结构域（PF00685）来鉴定小麦和六个相关物种（T. urartu、Ae. tauschii、T. turgidum、Z. mays、B. distachyon 和 Hordeum vulgare）中的 SOT 基因，尽管仅在小麦中进行了详细分析。将小麦基因映射到各个染色体上，并进行同线性和共线性分析。使用 NCBI 中的“保守结构域数据库”（CDD）搜索工具检查这些基因编码的蛋白质是否存在完整的 SOT 结构域。
在小麦中，鉴定出 107 个 TaSOT 基因，长度从 969 bp 到 7636 bp 不等，它们被映射到各个染色体上。还鉴定出 SSR（简单序列重复）、microRNAs、长非编码 RNA（lncRNA）及其靶位点存在于小麦 SOT 基因中。还对 SOT 蛋白进行了详细研究。通过小麦 RNA-seq 数据进行的 TaSOT 基因表达分析表明，这些基因参与了生长、发育以及对各种激素和生物/非生物胁迫的反应。
本研究的结果应该有助于进一步研究小麦和其他相关作物中 SOT 基因的功能特征。
