Li Rui, Zeng Cen, Wang Ping, Meng Xian-Li, Zeng Yong
Zhongguo Zhong Yao Za Zhi. 2014 Feb;39(4):704-9.
Since the polyjuice potion ingredient is complex, we need to develop an analysis method with well separation and high stability to perform qualitative analysis. After dividing chemical components of Mahuang Decoction into fat-soluble and water-soluble constituents by gradient extraction, GC-MS was used to analyze the chemical components of the ethyl acetate extraction. The results showed that forty compounds had been identified by NIST MS search 2.0 standard mass spectrometry Library and literatures. Next, UPLC-Q-TOF-MS was applied to idendify the chemical components of the water extraction. The results showed that thirty-nine compounds had been identified by MZmine-2.9.1, Isotope Pattern, fragmentation regularity of mass spectrometry and literatures. This experiment will provide evidences for elucidation of the effective substance in Mahuang decoction and can be used as a simple, shortcut method for analysis and identification for the polyjuice potion.
由于复方汤剂成分复杂，我们需要开发一种分离效果好且稳定性高的分析方法来进行定性分析。通过梯度萃取将麻黄汤的化学成分分为脂溶性成分和水溶性成分后，采用气相色谱-质谱联用（GC-MS）分析乙酸乙酯提取物的化学成分。结果表明，通过NIST MS search 2.0标准质谱图库和文献已鉴定出40种化合物。接下来，采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF-MS）鉴定水提取物的化学成分。结果表明，通过MZmine-2.9.1、同位素模式、质谱碎片规律和文献已鉴定出39种化合物。本实验将为阐明麻黄汤中的有效物质提供依据，可作为复方汤剂分析鉴定的一种简单、快捷的方法。
