Zhang Lin, Wang Chengying, Miao Dezu, Yuan Dan, Bi Kaishun, Yang Jingyu, Zhan Libin
a School of Pharmacy, Shenyang Pharmaceutical University , 110016 Shenyang , P.R. China.
Nat Prod Res. 2015;29(8):772-5. doi: 10.1080/14786419.2014.983104. Epub 2014 Nov 27.
Mahuang Fuzi Xixin (MFX), a classic recipe in traditional Chinese medicine, belongs to an exterior-relieving formula. For quality control of the MFX products, qualitative analysis using ultra-high performance liquid chromatography with diode-array detector-tandem mass spectrometry (UPLC-PDA-MS/MS) was undertaken. Six compounds from the MFX were simultaneously detected. Among them, astragalin and kaempferol 3-rutinoside were quantified through the UPLC-MS/MS method, while asarinin, sesamin, kakuol and methyleugenol were quantified through the UPLC-PDA method. This method can be applied to the quantitative determination of the six compounds in the MFX.
麻黄附子细辛汤（MFX）是中医经典方剂，属于解表剂。为对MFX产品进行质量控制，采用超高效液相色谱-二极管阵列检测器-串联质谱法（UPLC-PDA-MS/MS）进行定性分析。同时检测出了MFX中的6种化合物。其中，通过UPLC-MS/MS法对黄芪苷和山柰酚3-芸香糖苷进行定量，而通过UPLC-PDA法对细辛脂素、芝麻素、甲基丁香酚和细辛脑进行定量。该方法可用于MFX中6种化合物的定量测定。
