Lin Yu-Sheng, Chu Chin-Chou, Lin Jen-Jen, Chang Chien-Cheng, Wang Chun-Chieh, Wang Chiao-Yin, Tsui Po-Hsiang
Institude of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
Department of Applied Statistics and Information Science, Ming-Chuan University, Taoyuan, Taiwan.
Sci Rep. 2014 Sep 10;4:6316. doi: 10.1038/srep06316.
The planarian is widely used as a model for studying tissue regeneration. In this study, we used optical coherence tomography (OCT) for the real-time, high-resolution imaging of planarian tissue regeneration. Five planaria were sliced transversely to produce 5 head and 5 tail fragments. During a 2-week regeneration period, OCT images of the planaria were acquired to analyze the signal attenuation rates, intensity ratios, and image texture features (including contrast, correlation, homogeneity, energy, and entropy) to compare the primitive and regenerated tissues. In the head and tail fragments, the signal attenuation rates of the regenerated fragments decreased from -0.2 dB/μm to -0.05 dB/μm, between Day 1 and Day 6, and then increased to -0.2 dB/μm on Day 14. The intensity ratios decreased to approximately 0.8 on Day 6, and increased to between 0.8 and 0.9 on Day 14. The texture parameters of contrast, correlation, and homogeneity exhibited trends similar to the signal attenuation rates and intensity ratios during the planarian regeneration. The proposed OCT parameters might provide biological information regarding cell apoptosis and the formation of a mass of new cells during planarian regeneration. Therefore, OCT imaging is a potentially effective method for planarian studies.
涡虫被广泛用作研究组织再生的模型。在本研究中，我们使用光学相干断层扫描（OCT）对涡虫组织再生进行实时、高分辨率成像。将5只涡虫横向切片，产生5个头片段和5个尾片段。在为期2周的再生期内，采集涡虫的OCT图像，分析信号衰减率、强度比和图像纹理特征（包括对比度、相关性、均匀性、能量和熵），以比较原始组织和再生组织。在头片段和尾片段中，再生片段的信号衰减率在第1天到第6天从-0.2 dB/μm降至-0.05 dB/μm，然后在第14天升至-0.2 dB/μm。强度比在第6天降至约0.8，并在第14天升至0.8至0.9之间。对比度、相关性和均匀性的纹理参数在涡虫再生过程中呈现出与信号衰减率和强度比相似的趋势。所提出的OCT参数可能提供有关涡虫再生过程中细胞凋亡和大量新细胞形成的生物学信息。因此，OCT成像对于涡虫研究是一种潜在有效的方法。
