抗苗勒管激素和雄激素受体基因在形态学上高度分级的卵母细胞周围的人卵丘细胞中表达不足。
Genes for anti-Müllerian hormone and androgen receptor are underexpressed in human cumulus cells surrounding morphologically highly graded oocytes.
作者信息
Dević Pavlić Sanja, Tramišak Milaković Tamara, Panić Horvat Linda, Čavlović Kristina, Vlašić Hrvoje, Manestar Miljenko, Smiljan Severinski Neda, Radojčić Badovinac Anđelka
机构信息
Department of Biotechnology, University of Rijeka, Rijeka, Croatia.
Department of Obstetrics and Gynaecology, Clinical Hospital Centre Rijeka, Rijeka, Croatia.
出版信息
SAGE Open Med. 2019 Jul 16;7:2050312119865137. doi: 10.1177/2050312119865137. eCollection 2019.
OBJECTIVES
The aim of this study was to investigate the expression of genes crucial for the quality of the oocyte and whether expression levels of these genes in cumulus cells can be biological markers for the quality of the oocyte, zygote or embryo, or even for achievement of pregnancy after the assisted reproductive technology procedure. We examined the expression profile of the anti-Müllerian hormone (AMH) gene and its respective receptors: anti-Müllerian hormone receptor type 2 (AMHR2), follicle-stimulating hormone receptor (FSHR) and androgen receptor (AR) in cumulus cells (CCs) surrounding the oocyte, as well as AMH concentrations in follicular fluid of the associated follicle. The obtained gene expression levels were correlated with the morphological quality of the associated oocyte, zygote and embryo as well as with assisted reproductive technology outcome following the intracytoplasmic sperm injection procedure.
METHODS
This study involved 129 cumulus cells and 35 follicular fluid samples, taken from 58 patients undergoing the intracytoplasmic sperm injection procedure. Oocytes, zygotes and embryos were assessed for morphological quality. The relative gene expression of AMH, AMHR2, FSHR and AR was calculated using the delta-delta Ct method. Anti-Müllerian hormone concentrations in follicular fluids were measured by enzyme-linked immunosorbent assay.
RESULTS
The results yielded suggest a relationship between AMH, AR and oocyte morphology: AMH and AR gene expression levels in CCs surrounding morphologically optimal oocytes were significantly lower than in CCs surrounding oocytes with suboptimal morphology (p = 0.011 and p = 0.008, respectively). Statistically significant positive correlation was found between mRNA expression levels of AMH and FSHR (p < 0.001), AMH and AR (p = 0.001), AMHR2 and FSHR (p < 0.001), AMHR2 and AR (p < 0.001), as well as between FSHR and AR (p < 0.001).
CONCLUSION
Assessed results point to AMH and AR relation with oocyte maturity, but not with its fertilization potential, or with embryo quality.
目的
本研究旨在调查对卵母细胞质量至关重要的基因的表达情况，以及这些基因在卵丘细胞中的表达水平是否可作为卵母细胞、受精卵或胚胎质量的生物学标志物，甚至是辅助生殖技术程序后妊娠成功的生物学标志物。我们检测了卵母细胞周围卵丘细胞（CCs）中抗苗勒管激素（AMH）基因及其相应受体：抗苗勒管激素2型受体（AMHR2）、促卵泡激素受体（FSHR）和雄激素受体（AR）的表达谱，以及相关卵泡卵泡液中的AMH浓度。所获得的基因表达水平与相关卵母细胞、受精卵和胚胎的形态质量以及胞浆内单精子注射程序后的辅助生殖技术结果相关。
方法
本研究涉及从58例行胞浆内单精子注射程序的患者中采集的129个卵丘细胞和35份卵泡液样本。对卵母细胞、受精卵和胚胎进行形态质量评估。使用Δ-ΔCt法计算AMH、AMHR2、FSHR和AR的相对基因表达。通过酶联免疫吸附测定法测量卵泡液中的抗苗勒管激素浓度。
结果
结果表明AMH、AR与卵母细胞形态之间存在关联：形态学上最佳卵母细胞周围CCs中的AMH和AR基因表达水平显著低于形态学上欠佳卵母细胞周围的CCs（分别为p = 0.011和p = 0.008）。发现AMH与FSHR的mRNA表达水平之间（p < 0.001）、AMH与AR之间（p = 0.001）、AMHR2与FSHR之间（p < 0.001）、AMHR2与AR之间（p < 0.001）以及FSHR与AR之间（p < 0.001）存在统计学上显著的正相关。
结论
评估结果表明AMH和AR与卵母细胞成熟有关，但与卵母细胞的受精潜力或胚胎质量无关。
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