Demediuk P, Daly M P, Faden A I
Department of Neurology, University of California and Neurology Service, VA Medical Center, San Francisco 94121.
J Neurosci Res. 1989 May;23(1):95-106. doi: 10.1002/jnr.490230113.
Free fatty acids (FFA), phospholipid, and cholesterol levels were measured in spinal cord samples from rats subjected to low (25 g-cm), moderate (50 g-cm), or severe (100 g-cm) impact trauma to the T10 spinal segment. All degrees of injury caused early (15 min) declines in total phospholipids after trauma; phospholipid levels remained significantly below controls in rats subjected to moderate and severe injuries for up to 3 days, whereas phospholipids had returned to baseline values by 4 hr in the low injury group. Rapid and persistent decreases in cholesterol levels were observed for all injury groups. Severe trauma was associated with biphasic increases in FFA levels: levels were elevated at 5 and 15 min post-trauma and had declined by 30 min; a second elevation was observed at 1 hr, progressively increasing to reach a maximum at 24 hr, before declining over the next 6 days. Low and moderate injuries caused similar early total FFA increases; later increases were significantly smaller than in the severely injured group. Among the free fatty acids, significant increases were observed in palmitate, stearate, oleate, linoleate, linolenate, arachidonate, and docosahexaenoate. These findings indicate that traumatic spinal cord injury results in early, transient, postinjury membrane phospholipid hydrolysis, the magnitude of which is relatively independent of the severity of injury. More delayed and sustained lipid hydrolysis also occurs after trauma, the magnitude of which is related to the severity of injury.
在遭受T10脊髓节段低（25克力 - 厘米）、中（50克力 - 厘米）或重度（100克力 - 厘米）撞击创伤的大鼠脊髓样本中，测量了游离脂肪酸（FFA）、磷脂和胆固醇水平。所有损伤程度均导致创伤后早期（15分钟）总磷脂下降；中度和重度损伤的大鼠中，磷脂水平在长达3天的时间内仍显著低于对照组，而轻度损伤组的磷脂在4小时后已恢复至基线值。所有损伤组均观察到胆固醇水平迅速且持续下降。重度创伤与FFA水平的双相升高有关：创伤后5分钟和15分钟时水平升高，30分钟时下降；在1小时时观察到第二次升高，逐渐增加并在24小时时达到最大值，随后在接下来的6天内下降。轻度和中度损伤导致相似的早期总FFA升高；后期升高明显小于重度损伤组。在游离脂肪酸中，棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸、花生四烯酸和二十二碳六烯酸均显著增加。这些发现表明，创伤性脊髓损伤导致早期、短暂的损伤后膜磷脂水解，其程度相对独立于损伤的严重程度。创伤后还会发生更延迟和持续的脂质水解，其程度与损伤的严重程度有关。
