Center of Trauma Repair and Reconstruction of Chinese PLA and Department of Orthopedics of the 98th Hospital of Chinese PLA, Huzhou 313000, Zhejiang Province, China.
Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Neural Regen Res. 2013 Jan 25;8(3):233-43. doi: 10.3969/j.issn.1673-5374.2013.03.005.
This study established a dog model of acute multiple cauda equina constriction by experimental constriction injury (48 hours) of the lumbosacral central processes in dorsal root ganglia neurons. The repair effect of intrathecal injection of brain-derived neurotrophic factor with 15 mg encapsulated biodegradable poly(lactide-co-glycolide) nanoparticles on this injury was then analyzed. Dorsal root ganglion cells (L7) of all experimental dogs were analyzed using hematoxylin-eosin staining and immunohistochemistry at 1, 2 and 4 weeks following model induction. Intrathecal injection of brain-derived neurotrophic factor can relieve degeneration and inflammation, and elevate the expression of brain-derived neurotrophic factor in sensory neurons of compressed dorsal root ganglion. Simultaneously, intrathecal injection of brain-derived neurotrophic factor obviously improved neurological function in the dog model of acute multiple cauda equina constriction. Results verified that sustained intraspinal delivery of brain-derived neurotrophic factor encapsulated in biodegradable nanoparticles promoted the repair of histomorphology and function of neurons within the dorsal root ganglia in dogs with acute and severe cauda equina syndrome.
本研究通过实验性压迫损伤（48 小时）背根神经节神经元的腰骶部中央过程，建立了急性多发性马尾神经根受压的犬模型。然后分析了鞘内注射包裹在生物可降解聚（乳酸-共-乙醇酸）纳米粒子中的脑源性神经营养因子对这种损伤的修复作用。在模型诱导后 1、2 和 4 周，使用苏木精-伊红染色和免疫组织化学分析所有实验犬的背根神经节细胞（L7）。鞘内注射脑源性神经营养因子可减轻变性和炎症，并提高受压背根神经节感觉神经元中脑源性神经营养因子的表达。同时，鞘内注射脑源性神经营养因子明显改善了急性多发性马尾神经根受压犬模型的神经功能。结果证实，生物可降解纳米粒子包裹的脑源性神经营养因子的持续鞘内递送促进了急性严重马尾综合征犬背根神经节内神经元的组织形态和功能的修复。
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