Department of Physiology, İstanbul Faculty of Medicine, İstanbul University, İstanbul, Turkey.
Department of Biology, Faculty of Science, İstanbul University, İstanbul, Turkey.
Balkan Med J. 2012 Jun;29(2):188-96. doi: 10.5152/balkanmedj.2011.030. Epub 2012 Jun 1.
Flavonoids are an important group of recognized antioxidants in plants. Luteolin (LUT) is a natural flavonoid in the plant kingdom. This study was aimed to investigate the effects of the LUT in the liver, kidney and brain of pentylentetrazol (PTZ)-induced seizure and the relationship between nitric oxide synthases (iNOS, eNOS) and matrix metalloproteinases (MMP2, MMP9).
LUT (10 mg/kg) was given intraperitoneally during two weeks prior to seizure induction. A single dose PTZ 80 mg/kg i.p. was administered and seizures were observed and evaluated with regard to latency, frequency and stage for one hour.
Seizure frequen cy after PTZ administration was significantly decreased in LUT pretreated rats (p<0.05). An increase of immunhistochemical reactions of iNOS and MMP2, but a decrease of eNOS activity, were observed in rat hippocampus and peripheral tissues during the PTZ induced seizures. LUT pretreatment reversed the iNOS and MMP2 activity to the control levels and significantly increased the eNOS activity (p<0.001).
LUT seems to have an effective role in reducing the seizure frequency and a protective role on peripheral organ injury in animal models of seizure. The protective effect of LUT in seizures and the seizure induced peripheral tissue damage warrant further investigations.
类黄酮是植物中一种重要的已被认可的抗氧化剂。木樨草素（LUT）是植物王国中的一种天然类黄酮。本研究旨在探讨 LUT 对戊四氮（PTZ）诱导的癫痫发作时肝脏、肾脏和大脑的影响，以及一氧化氮合酶（iNOS、eNOS）与基质金属蛋白酶（MMP2、MMP9）之间的关系。
在诱导癫痫发作前两周，通过腹腔内注射给予 LUT（10mg/kg）。腹腔内给予单次剂量的 80mg/kg PTZ，并观察和评估 1 小时内的潜伏期、频率和阶段的癫痫发作。
与未给予 LUT 预处理的大鼠相比，给予 LUT 预处理的大鼠在 PTZ 给药后的癫痫发作频率显著降低（p<0.05）。在 PTZ 诱导的癫痫发作期间，大鼠海马体和外周组织中观察到 iNOS 和 MMP2 的免疫组织化学反应增加，但 eNOS 活性降低。LUT 预处理将 iNOS 和 MMP2 活性逆转至对照水平，并显著增加 eNOS 活性（p<0.001）。
LUT 似乎在降低癫痫发作频率和保护动物模型中癫痫发作时外周器官损伤方面具有有效作用。LUT 在癫痫发作和癫痫诱导的外周组织损伤中的保护作用需要进一步研究。
