Hanin V, Naharisoa H, Sarrade V, Calas B
Centre de Recherches de Biochimie Macromoléculaire, UPR 8402 CNRS et U249 INSERM, Montpellier, France.
Pept Res. 1989 Nov-Dec;2(6):367-72.
Polyacrylamide-based solid-phase supports were used in the production of a synthetic peptide analogous to amino acid sequence 1-9 from bovine aortic actin. Different peptidyl-resins were prepared and directly used to induce an antibody response in rabbits. Antisera that recognized the resin-bound peptide were compared. Some of them reacted with native aortic actin; this response was highly specific for the smooth muscle alpha actin isotype. We also immunized rabbits with the 1-9 peptide linked to bovine serum albumin. In this case, antisera were less reactive with native aortic actin, and cross-reacted with the two closely related smooth muscle alpha and macrophage actin isotypes. This method provides a rapid and simple procedure for generating peptide immunogens and antibodies to them, by the combination of peptidyl-resin immunizations and resin-bound peptide immunoassays.
基于聚丙烯酰胺的固相载体被用于合成一种类似于牛主动脉肌动蛋白氨基酸序列1 - 9的肽。制备了不同的肽基树脂，并直接用于诱导兔的抗体反应。比较了识别树脂结合肽的抗血清。其中一些与天然主动脉肌动蛋白发生反应；这种反应对平滑肌α肌动蛋白亚型具有高度特异性。我们还用与牛血清白蛋白连接的1 - 9肽免疫兔子。在这种情况下，抗血清与天然主动脉肌动蛋白的反应性较低，并且与两种密切相关的平滑肌α和巨噬细胞肌动蛋白亚型发生交叉反应。通过肽基树脂免疫和树脂结合肽免疫测定相结合，该方法为产生肽免疫原及其抗体提供了一种快速简单的程序。
