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肌动蛋白抗体。平滑肌肌动蛋白亚型特异性抗体的制备与表征。

Actin antibodies. Preparation and characterization of antibodies specific for smooth-muscle actin isoforms.

作者信息

Cavadore J C, Martin F, Calas B, Mery J, Berta P, Benyamin Y, Roustan C

出版信息

Biochem J. 1987 Feb 15;242(1):51-4. doi: 10.1042/bj2420051.

DOI:10.1042/bj2420051
PMID:3593248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147662/
Abstract

We have determined the specificity of sera elicited by glutaraldehyde-stabilized bovine aortic actin. This modification induces a high titre of antibodies directed against the N-terminal (residues 1-39) and the C-terminal region of smooth-muscle actins. The crude antisera were purified on peptide (corresponding to the 1-9 or 1-8 N-terminal sequences of smooth-muscle isoactins)-polyacrylic-resin columns. By fractionating the antisera we obtained oligoclonal antibody populations specific for each isoactin.

摘要

我们已经确定了由戊二醛稳定的牛主动脉肌动蛋白引发的血清的特异性。这种修饰诱导产生了针对平滑肌肌动蛋白N端（第1 - 39位氨基酸残基）和C端区域的高滴度抗体。粗抗血清在肽（对应于平滑肌同工肌动蛋白的第1 - 9或1 - 8 N端序列）-聚丙烯树脂柱上进行纯化。通过对抗血清进行分级分离，我们获得了对每种同工肌动蛋白具有特异性的寡克隆抗体群体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/1147662/2b724cb868cf/biochemj00261-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/1147662/82eb2ccebef8/biochemj00261-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/1147662/2b724cb868cf/biochemj00261-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/1147662/82eb2ccebef8/biochemj00261-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01dd/1147662/2b724cb868cf/biochemj00261-0061-a.jpg

相似文献

1
Actin antibodies. Preparation and characterization of antibodies specific for smooth-muscle actin isoforms.肌动蛋白抗体。平滑肌肌动蛋白亚型特异性抗体的制备与表征。
Biochem J. 1987 Feb 15;242(1):51-4. doi: 10.1042/bj2420051.
2
Production of oligoclonal antibodies directed to the N-terminal of smooth muscle alpha actin using peptidyl-polyacrylic resins as direct immunogens.使用肽基聚丙烯酸树脂作为直接免疫原生产针对平滑肌α肌动蛋白N端的寡克隆抗体。
Pept Res. 1989 Nov-Dec;2(6):367-72.
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Identification of L-cell growth stimulating antibody as anti-actin.
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A vascular smooth muscle alpha-isoactin biosynthetic intermediate in BC3H1 cells. Identification of acetylcysteine at the NH2 terminus.BC3H1细胞中的一种血管平滑肌α-异肌动蛋白生物合成中间体。NH2末端乙酰半胱氨酸的鉴定。
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引用本文的文献

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Comparative study of invertebrate actins: antigenic cross-reactivity versus sequence variability.无脊椎动物肌动蛋白的比较研究：抗原交叉反应性与序列变异性
J Muscle Res Cell Motil. 1989 Apr;10(2):135-42. doi: 10.1007/BF01739969.
2
Structural and functional variations in skeletal-muscle and scallop muscle actins.骨骼肌肌动蛋白和扇贝肌肌动蛋白的结构与功能变异
Biochem J. 1988 Dec 15;256(3):853-9. doi: 10.1042/bj2560853.

本文引用的文献

1
Purification of muscle actin.肌肉肌动蛋白的纯化
Methods Enzymol. 1982;85 Pt B:164-81. doi: 10.1016/0076-6879(82)85020-9.
2
Actin typing on total cellular extracts: a highly sensitive protein-chemical procedure able to distinguish different actins.全细胞提取物的肌动蛋白分型：一种能够区分不同肌动蛋白的高灵敏度蛋白质化学方法。
Eur J Biochem. 1981 Jan;113(3):595-603. doi: 10.1111/j.1432-1033.1981.tb05104.x.
3
Actin and tropomyosin variants in smooth muscles. Dependence on tissue type.平滑肌中的肌动蛋白和原肌球蛋白变体。对组织类型的依赖性。
J Biol Chem. 1984 Dec 10;259(23):14383-8.
4
Correlation between segmental mobility and the location of antigenic determinants in proteins.蛋白质中片段流动性与抗原决定簇位置之间的相关性。
Nature. 1984;311(5982):123-6. doi: 10.1038/311123a0.
5
Induction by chemically modified actin derivatives of antibody specificity. A relation between modified sites and antibody interactions with monomeric and filamentous actins.通过化学修饰的肌动蛋白衍生物诱导抗体特异性。修饰位点与抗体与单体和丝状肌动蛋白相互作用之间的关系。
FEBS Lett. 1983 Aug 22;160(1-2):41-5. doi: 10.1016/0014-5793(83)80932-6.
6
Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.用于检测肽固相合成中游离末端氨基的颜色测试。
Anal Biochem. 1970 Apr;34(2):595-8. doi: 10.1016/0003-2697(70)90146-6.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
Cleavage of actin by thrombin.凝血酶对肌动蛋白的切割作用。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2208-11. doi: 10.1073/pnas.71.6.2208.
9
Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. A quantitative determination of the two actin isoforms.骨骼肌和心肌肌动蛋白蛋白在各种横纹肌组织和细胞中的同时表达。两种肌动蛋白异构体的定量测定。
J Biol Chem. 1986 Feb 5;261(4):1838-43.
10
Structural variations in actins. Biochemical and immunological tools for probing the structure of rabbit skeletal-muscle and bovine aortic actins.肌动蛋白的结构变异。用于探究兔骨骼肌和牛主动脉肌动蛋白结构的生化及免疫学工具。
Biochem J. 1985 Oct 15;231(2):363-8. doi: 10.1042/bj2310363.