Zhu Xiaoyang, Lei Hehua, Wu Junfang, Li Jia V, Tang Huiru, Wang Yulan
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences , Wuhan 430071, P. R. China.
J Proteome Res. 2014 Oct 3;13(10):4436-45. doi: 10.1021/pr500770x. Epub 2014 Sep 23.
Salmonella typhimurium is a bacterial pathogen that poses a great threat to humans and animals. In order to discover hosts' responses to S. typhimurium infection, we collected and analyzed biofluids and organ tissues from mice which had ingested S. typhimurium. We employed (1)H NMR spectroscopy coupled with multivariate data analysis and immunological techniques. The results indicate that infection leads to a severe impact on mice spleen and ileum, which are characterized by splenomegaly and edematous villi, respectively. We found that increased levels of itaconic acid were correlated with the presence of splenomegaly during infection and may play an important role in Salmonella-containing vacuole acidification. In addition, metabonomic analyses of urine displayed the development of salmonellosis in mice, which is characterized by dynamic changes in energy metabolism. Furthermore, we found that the presence of S. typhimurium activated an anti-oxidative response in infected mice. We also observed changes in the gut microbial co-metabolites (hippurate, TMAO, TMA, methylamine). This investigation sheds much needed light on the host-pathogen interactions of S. typhimurium, providing further information to deepen our understanding of the long co-evolution process between hosts and infective bacteria.
鼠伤寒沙门氏菌是一种对人类和动物构成巨大威胁的细菌病原体。为了发现宿主对鼠伤寒沙门氏菌感染的反应，我们收集并分析了摄入鼠伤寒沙门氏菌的小鼠的生物流体和器官组织。我们采用了核磁共振氢谱结合多变量数据分析和免疫学技术。结果表明，感染对小鼠脾脏和回肠造成了严重影响，分别表现为脾肿大和绒毛水肿。我们发现，感染期间衣康酸水平的升高与脾肿大的存在相关，并且可能在含沙门氏菌液泡的酸化过程中发挥重要作用。此外，尿液的代谢组学分析显示了小鼠沙门氏菌病的发展，其特征是能量代谢的动态变化。此外，我们发现鼠伤寒沙门氏菌的存在激活了受感染小鼠的抗氧化反应。我们还观察到肠道微生物共代谢产物（马尿酸盐、氧化三甲胺、三甲胺、甲胺）的变化。这项研究为鼠伤寒沙门氏菌的宿主-病原体相互作用提供了急需的见解，提供了进一步的信息，以加深我们对宿主与感染性细菌之间长期共同进化过程的理解。
