Everest P, Allen J, Papakonstantinopoulou A, Mastroeni P, Roberts M, Dougan G
Department of Biochemistry, Imperial College of Science Technology, and Medicine, London, United Kingdom.
J Immunol. 1997 Aug 15;159(4):1820-7.
Mice harboring mutations in the IL-4 gene (IL-4(-/-)) were infected with a range of Salmonella typhimurium HWSH derivatives using different routes of infection. Compared with IL-4(+/+) mice, IL-4(-/-) mice exhibited a delayed time to death following infection with wild-type S. typhimurium HWSH. Groups of IL-4(+/+) mice infected with S. typhimurium HWSH purE, a less virulent derivative, showed sporadic deaths and harbored micro- or macroabscesses in their tissues, particularly associated with the liver. However, IL-4(-/-) mice infected with similar doses of S. typhimurium HWSH purE bacteria were resistant to killing and failed to develop detectable abscesses. Abscess formation in IL-4(-/-) mice could be induced by i.v. administration of rIL-4 during the S. typhimurium HWSH purE infection. The immune response in both IL-4(-/-) and IL-4(+/+) mice was of the Th1-type. Viable salmonella bacteria could be found associated with abscesses. Both IL-4(-/-) and IL-4(+/+) mice were resistant to killing by S. typhimurium aroA.
利用不同的感染途径，用一系列鼠伤寒沙门氏菌HWSH衍生物感染白细胞介素4基因发生突变的小鼠（IL-4(-/-)）。与IL-4(+/+)小鼠相比，IL-4(-/-)小鼠在感染野生型鼠伤寒沙门氏菌HWSH后死亡时间延迟。用毒性较低的衍生物鼠伤寒沙门氏菌HWSH purE感染的IL-4(+/+)小鼠组出现散发性死亡，并且其组织中存在微脓肿或大脓肿，尤其是与肝脏相关。然而，用相似剂量的鼠伤寒沙门氏菌HWSH purE细菌感染的IL-4(-/-)小鼠对致死有抗性，并且未形成可检测到的脓肿。在鼠伤寒沙门氏菌HWSH purE感染期间，通过静脉注射重组白细胞介素4（rIL-4）可诱导IL-4(-/-)小鼠形成脓肿。IL-4(-/-)和IL-4(+/+)小鼠的免疫反应均为Th1型。在脓肿中可发现存活的沙门氏菌。IL-4(-/-)和IL-4(+/+)小鼠均对鼠伤寒沙门氏菌aroA的致死有抗性。
