Hadrich Inès, Neji Sourour, Makni Fattouma, Ayadi Ali, Elloumi Moez, Ranque Stéphane
Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
Service d'Onco-hématologie, CHU Hedi-Chaker, 3029 Sfax, Tunisia.
J Med Microbiol. 2014 Dec;63(Pt 12):1584-1589. doi: 10.1099/jmm.0.076000-0. Epub 2014 Sep 10.
There are limited data on in vitro susceptibility testing of echinocandins against Aspergillus species. The objective of this study was to describe the phenotypes of Aspergillus flavus observed on exposure to caspofungin in vitro and to test whether these phenotypes were associated with A. flavus genotypes. The caspofungin MICs of 37 A. flavus clinical isolates collected from 14 patients with invasive aspergillosis were determined using Etest assays. Caspofungin MICs ranged from 0.012 to 0.064 mg l(-1); the modal MIC was 0.023 mg l(-1) and the MIC₅₀ and MIC₉₀ were 0.032 and 0.064 mg l(-1), respectively. A clear end point was noted in 24 (65 %) isolates, whereas seven (19 %) displayed a trailing effect and six (16 %) showed paradoxical growth when exposed to caspofungin. In these A. flavus isolates, the absence of a significant population structure or genetic differentiation indicated that trailing or paradoxical growth phenotypes were independent of microsatellite genotype.
关于棘白菌素对曲霉属菌种的体外药敏试验数据有限。本研究的目的是描述黄曲霉在体外接触卡泊芬净时观察到的表型，并测试这些表型是否与黄曲霉基因型相关。使用Etest法测定了从14例侵袭性曲霉病患者中收集的37株黄曲霉临床分离株的卡泊芬净MIC。卡泊芬净MIC范围为0.012至0.064 mg l(-1)；众数MIC为0.023 mg l(-1)，MIC₅₀和MIC₉₀分别为0.032和0.064 mg l(-1)。24株（65%）分离株观察到明确的终点，而7株（19%）表现出拖尾效应，6株（16%）在接触卡泊芬净时出现矛盾生长。在这些黄曲霉分离株中，不存在显著的群体结构或遗传分化，表明拖尾或矛盾生长表型与微卫星基因型无关。
