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大肠杆菌中的蛋白质聚集：营养密度的短期和长期影响

Protein aggregation in E. coli : short term and long term effects of nutrient density.

作者信息

Baig Ulfat I, Bhadbhade Bharati J, Mariyam Dincy, Watve Milind G

机构信息

Department of Microbiology, Abasaheb Garware College, Pune, Maharashtra, India; Department of Biology, Indian Institute of Science Education and Research, Pune, Maharashtra, India.

Department of Microbiology, Abasaheb Garware College, Pune, Maharashtra, India.

出版信息

PLoS One. 2014 Sep 11;9(9):e107445. doi: 10.1371/journal.pone.0107445. eCollection 2014.

DOI:10.1371/journal.pone.0107445
PMID:25210787
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4161400/
Abstract

During exponential growth some cells of E. coli undergo senescence mediated by asymmetric segregation of damaged components, particularly protein aggregates. We showed previously that functional cell division asymmetry in E. coli was responsive to the nutritional environment. Short term exposure as well as long term selection in low calorie environments led to greater cell division symmetry and decreased frequency of senescent cells as compared to high calorie environments. We show here that long term selection in low nutrient environment decreased protein aggregation as revealed by fluorescence microscopy and proportion of insoluble proteins. Across selection lines protein aggregation was correlated significantly positively with the RNA content, presumably indicating metabolic rate. This suggests that the effects of caloric restriction on cell division symmetry and aging in E. coli may work via altered protein handling mechanisms. The demonstrable effects of long term selection on protein aggregation suggest that protein aggregation is an evolvable phenomenon rather than being a passive inevitable process. The aggregated proteins progressively disappeared on facing starvation indicating degradation and recycling demonstrating that protein aggregation is a reversible process in E. coli.

摘要

在指数生长期间，一些大肠杆菌细胞会经历由受损成分（特别是蛋白质聚集体）的不对称分离介导的衰老过程。我们之前表明，大肠杆菌中功能性细胞分裂不对称对营养环境有反应。与高热量环境相比，在低热量环境中的短期暴露以及长期选择导致更大的细胞分裂对称性和衰老细胞频率降低。我们在此表明，通过荧光显微镜和不溶性蛋白质比例揭示，在低营养环境中的长期选择降低了蛋白质聚集。在各个选择系中，蛋白质聚集与RNA含量显著正相关，这大概表明了代谢率。这表明热量限制对大肠杆菌细胞分裂对称性和衰老的影响可能通过改变蛋白质处理机制起作用。长期选择对蛋白质聚集的明显影响表明蛋白质聚集是一种可进化的现象，而不是一个被动的必然过程。面对饥饿时，聚集的蛋白质逐渐消失，表明其降解和再循环，这证明蛋白质聚集在大肠杆菌中是一个可逆过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/e69f758897a6/pone.0107445.g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/1144af16c4ef/pone.0107445.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/e9d5fcb2d6a8/pone.0107445.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/f162e910da72/pone.0107445.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/e69f758897a6/pone.0107445.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dcf/4161400/cb3e8bde1a3d/pone.0107445.g001.jpg
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