Pereira Joice N B, Mari Renata B, Stabille Sandra R, de Faria Haroldo G, Mota Thais F M, Ferreira Walter M
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
Universidade Estadual Paulista ?Júlio de Mesquita Filho?, Campus Litoral Paulista, São Vicente, SP, Brasil.
An Acad Bras Cienc. 2014 Sep;86(3):1471-81. doi: 10.1590/0001-3765201420130052.
Aging is a biologic process characterized by progressive damage of structures and functions of organic systems. In gastrointestinal tract, it can involve enteric nervous system, which plays an important role in digestion and absorption of nutrients, causing hastening of intestinal transit thus reducing its absorptive function. Caloric restriction has been used in several studies with the intention of delaying deleterious effects of aging. This study aimed to evaluate the effects of caloric restriction on myenteric neurons of ileum by aging in rats. 30 Wistar rats were grouped as follows: GI (animals aged 6 months fed with normal diet), GII (animals aged 18 months fed with normal diet) and GIII (animals aged 18 months subject to 31% of caloric restriction). The rats of the GI group were euthanized at 6 months of age and after experimental period of 12 months animals of the group GII and GIII were euthanized, the ileum of all groups were collected, measured and processed by NADPH-dp and Acetylcholinesterase. Quantitative analysis of neurons revealed that aging promotes the increasing of myenteric neurons NADPH-dp and reduces Acetylcholinesterase neuronal population. However, in the cellular profile area, were not observed significant differences between the groups. The caloric restriction has been efficient and can be used preventively because it minimizes quantitative changes associated with aging on ileum myenteric plexuses.
衰老 是一个生物学过程，其特征是有机系统的结构和功能逐渐受损。在胃肠道中，衰老会影响肠神经系统，而肠神经系统在营养物质的消化和吸收中起重要作用，会导致肠道转运加快，从而降低其吸收功能。在多项研究中，人们采用热量限制的方法，以期延缓衰老的有害影响。本研究旨在评估热量限制对衰老大鼠回肠肌间神经元的影响。30只Wistar大鼠被分为以下几组：GI组（6月龄正常饮食的动物）、GII组（18月龄正常饮食的动物）和GIII组（18月龄接受31%热量限制的动物）。GI组大鼠在6月龄时实施安乐死，GII组和GIII组动物在经过12个月的实验期后实施安乐死，收集所有组的回肠，进行测量，并通过还原型辅酶II-黄递酶（NADPH-dp）和乙酰胆碱酯酶进行处理。对神经元的定量分析显示，衰老会促使肌间神经元的NADPH-dp增加，并减少乙酰胆碱酯酶神经元数量。然而，在细胞轮廓区域，各组之间未观察到显著差异。热量限制是有效的，并且可以预防性使用，因为它能将与衰老相关的回肠肌间神经丛的定量变化降至最低。
