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肠道运动功能障碍在 Goto-Kakizaki 大鼠：肌间神经丛的作用。

Intestinal Motility Dysfunction in Goto-Kakizaki Rats: Role of the Myenteric Plexus.

机构信息

Interdisciplinary Post-Graduate Program in Health Science, Institute of Physical Activity and Sports Sciences, Cruzeiro do Sul University, Rua Galvão Bueno, 868, Liberdade, São Paulo 01506-000, Brazil.

Butantan Institute, São Paulo 05585-000, Brazil.

出版信息

Cells. 2024 Sep 28;13(19):1626. doi: 10.3390/cells13191626.

DOI:10.3390/cells13191626
PMID:39404390
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11475219/
Abstract

UNLABELLED

Diabetes mellitus is associated with changes in intestinal morphology and the enteric nervous system. We previously reported constipation in Goto-Kakizaki (GK) rats, a non-obese model for type 2 diabetes mellitus.

AIM

The morpho-quantitative analysis of myenteric plexus neurons in the small and large intestines of 120-day-old male GK rats was investigated.

METHODS

The diabetes was confirmed by high fasting blood glucose levels. The myenteric plexus was evaluated through wholemount immunofluorescence. The morpho-quantitative analyses included evaluating neuronal density (neurons per ganglion) of the total neuronal population, the cholinergic and nitrergic subpopulations, and enteric glial cells per ganglion. The cell body area of 100 neurons per segment per animal was measured.

RESULTS

The total neurons and nitrergic subpopulation were unaltered in the GK rats' small and large intestines. The cholinergic subpopulation exhibited decreased density in the three segments of the small intestine and an increased number in the proximal colon of the GK rats. The number of enteric glial cells increased in the ileum of the GK rats, which could indicate enteric gliosis caused by the intestinal inflammatory state. The area of the cell body was increased in the total neuronal population of the jejunum and ileum of the GK rats. Frequency histograms of the cell body area distribution revealed the contribution of cholinergic neurons to larger areas in the jejunum and nitrergic neurons in the ileum.

CONCLUSION

The constipation previously reported in GK rats might be explained by the decrease in the density of cholinergic neurons in the small intestine of this animal model.

摘要

目的

研究 120 日龄雄性 GK 大鼠（2 型糖尿病非肥胖模型）小、大肠肌间神经丛神经元的形态定量分析。

方法

高血糖确认糖尿病，全肠系膜铺片免疫荧光法评估肌间神经丛，对总神经元、胆碱能和氮能亚群以及每个神经节的肠胶质细胞进行形态定量分析，测量每个动物每个节段 100 个神经元的胞体面积。

结果

小、大肠中 GK 大鼠的总神经元和氮能亚群无变化。胆碱能亚群在小肠的三个节段中密度降低，而在 GK 大鼠的近端结肠中数量增加。GK 大鼠回肠中的肠胶质细胞数量增加，这可能表明肠道炎症状态引起的肠胶质增生。GK 大鼠空肠和回肠的总神经元群体的细胞体面积增加。细胞体面积分布的频率直方图显示，空肠中的胆碱能神经元和回肠中的氮能神经元的面积较大。

结论

以前报道的 GK 大鼠便秘可能与该动物模型小肠内胆碱能神经元密度降低有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/5bfb07b39880/cells-13-01626-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/3ee5453c3a1d/cells-13-01626-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/66aea9031972/cells-13-01626-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/838d4bc2f418/cells-13-01626-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/65d43893d54b/cells-13-01626-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/fa2e2bdb1cb2/cells-13-01626-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/f66392445a54/cells-13-01626-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/defe090ad66e/cells-13-01626-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/f49a48ea3244/cells-13-01626-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/5bfb07b39880/cells-13-01626-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/3ee5453c3a1d/cells-13-01626-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/66aea9031972/cells-13-01626-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/838d4bc2f418/cells-13-01626-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/65d43893d54b/cells-13-01626-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/fa2e2bdb1cb2/cells-13-01626-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/f66392445a54/cells-13-01626-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/defe090ad66e/cells-13-01626-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/f49a48ea3244/cells-13-01626-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9377/11475219/5bfb07b39880/cells-13-01626-g009.jpg

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