Sun Bingyun
Department of Chemistry and Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
Proteomics. 2015 Mar;15(5-6):1152-63. doi: 10.1002/pmic.201400300. Epub 2014 Oct 28.
Pluripotent stem cells are a unique cell type with promising potential in regenerative and personalized medicine. Yet the difficulty to understand and coax their seemingly stochastic differentiation and spontaneous self-renewal have largely limited their clinical applications. A call has been made by numerous researchers for a better characterization of surface proteins on these cells, in search of biomarkers that can dictate developmental stages and lineage specifications, and can help formulate mechanistic insight of stem-cell fate choices. In the past two decades, proteomics has gained significant recognition in profiling surface proteins at high throughput. This review will summarize the impact of these studies on stem-cell biology, and discuss the used proteomic techniques. A systematic comparison of all the techniques and their results is also attempted here to help reveal pros, cons, and the complementarity of the existing methods. This awareness should assist in selecting suitable strategies for stem-cell related research, and shed light on technical improvements that can be explored in the future.
多能干细胞是一种独特的细胞类型，在再生医学和个性化医疗方面具有广阔的应用前景。然而，理解并引导它们看似随机的分化和自发的自我更新存在困难，这在很大程度上限制了它们的临床应用。众多研究人员呼吁更好地表征这些细胞上的表面蛋白，寻找能够指示发育阶段和谱系特化的生物标志物，并有助于形成对干细胞命运选择的机制性认识。在过去二十年中，蛋白质组学在高通量分析表面蛋白方面获得了广泛认可。本综述将总结这些研究对干细胞生物学的影响，并讨论所使用的蛋白质组学技术。本文还尝试对所有技术及其结果进行系统比较，以帮助揭示现有方法的优缺点及互补性。这种认识将有助于为干细胞相关研究选择合适的策略，并为未来可探索的技术改进提供思路。
