Hatayama Kouta
Sagami Chemical Research Institute, Ayase, Kanagawa 252-1193, Japan.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):3976-3982. doi: 10.1099/ijs.0.067439-0. Epub 2014 Sep 11.
A bacterial strain, designated GAU11(T), was isolated from soil in Japan. Cells of the strain were Gram-stain-negative, aerobic, non-motile rods. The 16S rRNA gene sequence of strain GAU11(T) showed high similarity to those of Comamonas zonglianii BF-3(T) (98.8 %), Pseudacidovorax intermedius CC21(T) (96.4 %), Acidovorax caeni R-24608(T) (96.2 %), Alicycliphilus denitrificans K601(T) (96.2 %), Pseudorhodoferax soli TBEA3(T) (95.9 %) and Comamonas terrigena LMG 1253(T) (95.9 %). Strain GAU11(T) contained ubiquinone 8 as the sole ubiquinone and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. Its major cellular fatty acids were C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The DNA G+C content of strain GAU11(T) was 68.2 mol%. The DNA-DNA relatedness between strain GAU11(T) and C. zonglianii DSM 22523(T) was 52 or 68 % (reciprocal value). Phenotypic characterization indicated that strain GAU11(T) represents a member of the genus Comamonas, but at the same time distinguished it from C. zonglianii DSM 22523(T). From polyphasic characterization, this strain should be classified as representing a novel species of the genus Comamonas, for which the name Comamonas humi sp. nov. (type strain GAU11(T) = JCM 19903(T) = DSM 28451(T)) is proposed.
从日本土壤中分离出一株细菌，命名为GAU11(T)。该菌株细胞革兰氏染色阴性，好氧，无运动性杆菌。菌株GAU11(T)的16S rRNA基因序列与宗联丛毛单胞菌BF-3(T)（98.8%）、中间假食酸菌CC21(T)（96.4%）、嗜泥嗜酸菌R-24608(T)（96.2%）、反硝化脂环酸杆菌K601(T)（96.2%）、土壤假红环菌TBEA3(T)（95.9%）和土生丛毛单胞菌LMG 1253(T)（95.9%）的序列具有高度相似性。菌株GAU11(T)含有泛醌8作为唯一的泛醌，二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油作为主要极性脂质。其主要细胞脂肪酸为C16 : 0、C18 : 1ω7c和特征性总和3（C16 : 1ω7c和/或异-C15 : 0 2-OH）。菌株GAU11(T)的DNA G+C含量为68.2 mol%。菌株GAU11(T)与宗联丛毛单胞菌DSM 22523(T)之间的DNA-DNA相关性为52%或68%（ reciprocal值）。表型特征表明，菌株GAU11(T)代表丛毛单胞菌属的一个成员，但同时又与宗联丛毛单胞菌DSM 22523(T)不同。综合多相特征分析，该菌株应归类为丛毛单胞菌属的一个新物种，为此提出名为Comamonas humi sp. nov.（模式菌株GAU11(T) = JCM 19903(T) = DSM 28451(T)）。
