Zhu Daochen, Xie Changxiao, Huang Ying, Sun Jianzhong, Zhang Weimin
State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, PR China.
School of Environmental Engineering, Jiangsu University, 301 Xuefu Road Zhenjiang 212013, PR China.
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4141-4146. doi: 10.1099/ijs.0.066688-0. Epub 2014 Sep 21.
A Gram-stain-negative bacterium, designated SP-35(T), was isolated from compost and was subjected to a taxonomic study. This isolate was short-rod-shaped and non-spore-forming. Phylogenetic analysis based on 16S rRNA sequence comparison indicated the isolate was related to the genus Comamonas. 16S rRNA gene sequence analysis showed that its closest neighbours were the type strains Comamonas odontotermitis Dant 3-8(T) (96.8 % similarity), Comamonas testosteroni DSM 50244(T) (96.5 %), Comamonas guangdongensis CY01(T) (95.9 %) and Comamonas composti YY287(T) (95.6 %). Using phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics we could clearly distinguish strain SP-35(T) from type strains of the genus Comamonas. The genomic DNA G+C content of strain SP-35(T) was 63.1 mol%. The predominant cellular fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidlyglycerol. Differences in phenotypic and phylogenetic characteristics support the classification of strain SP-35(T) as a representative of a novel species in the genus Comamonas, for which the name Comamonas serinivorans sp. nov. is proposed. The type strain is SP-35(T) ( = DSM 26136(T) = JCM 18194(T)).
从堆肥中分离出一株革兰氏阴性菌，命名为SP-35(T)，并对其进行了分类学研究。该分离株为短杆状，不形成芽孢。基于16S rRNA序列比较的系统发育分析表明，该分离株与丛毛单胞菌属相关。16S rRNA基因序列分析显示，其最接近的亲缘菌株是齿白蚁丛毛单胞菌Dant 3-8(T)（相似度96.8%）、睾丸酮丛毛单胞菌DSM 50244(T)（96.5%）、广东丛毛单胞菌CY01(T)（95.9%）和堆肥丛毛单胞菌YY287(T)（95.6%）。通过系统发育分析、DNA-DNA杂交、脂肪酸组成数据以及一系列生理生化特征，我们能够清楚地将菌株SP-35(T)与丛毛单胞菌属的模式菌株区分开来。菌株SP-35(T)的基因组DNA G+C含量为63.1 mol%。主要的细胞脂肪酸为C16 : 0、C17 : 0环丙烷、特征性总和3（C16 : 1ω6c和/或C16 : 1ω7c）和特征性总和8（C18 : 1ω6c和/或C18 : 1ω7c）。主要的极性脂质为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。表型和系统发育特征的差异支持将菌株SP-35(T)分类为丛毛单胞菌属一个新物种代表，为此提出新名称丝氨酸食丛毛单胞菌（Comamonas serinivorans sp. nov.）。模式菌株为SP-35(T)（=DSM 26136(T)=JCM 18194(T)）。
