Department of Life Science, Graduate School, Kyonggi University, 154-42 Gwanggyosan-ro Yeongtong-gu, Suwon-si, Gyeonggi-do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004681.
A novel bacterial strain, EJ-4, isolated from stream water collected at Seo-ho in Suwon, Republic of Korea, was characterized based on a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EJ-4 belonged to the genus . The isolate is Gram-stain-negative, non-motile, aerobic, rod-shaped and forms pale yellow colonies on trypticase soy agar. The optimal growth of this strain was observed aerobically at 30 °C, pH 7 and 0.5 % NaCl. The major fatty acids were summed feature 3 (C 7 and/or C 6; 39.7 %) and C (32.0 %). The G+C content of strain EJ-4 was 58.4mol %. The average nucleotide identity and digital DNA-DNA hybridization values between strain EJ-4 and were 91.8 and 31.2 %, respectively. The major polar lipids detected in the isolate were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant isoprenoid quinone was ubiquinone-8. Based on the results of polyphasic taxonomic analysis of strain EJ-4, we describe a novel species of the genus , for which the name sp. nov. has been proposed, with EJ-4 (=KCTC 82074=JCM 34179=KEMB 1602-279) as the type strain.
从韩国水原市 Seo-ho 采集的溪流水中分离到的一株新型细菌菌株 EJ-4，通过多相分类学方法进行了表征。基于 16S rRNA 基因序列的系统发育分析表明，菌株 EJ-4 属于 属。该分离株革兰氏阴性，非运动性，需氧，杆状，在胰蛋白酶大豆琼脂上形成浅黄色菌落。该菌株的最佳生长条件为需氧、30°C、pH 7 和 0.5%NaCl。主要脂肪酸为(summed feature 3，C7 和/或 C6；39.7%)和 C (32.0%)。菌株 EJ-4 的 G+C 含量为 58.4mol%。菌株 EJ-4 与 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 91.8%和 31.2%。在该分离株中检测到的主要极性脂质是双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。主要的异戊二烯醌是泛醌-8。基于菌株 EJ-4 的多相分类学分析结果，我们描述了 属的一个新种，建议将其命名为 sp. nov.，其模式菌株为 EJ-4(=KCTC 82074=JCM 34179=KEMB 1602-279)。
