荔枝叶的化学成分、抗氧化及抗伤害感受特性

Chemical composition, antioxidant and antinociceptive properties of Litchi chinensis leaves.

作者信息

Castellain Rosana C L, Gesser Marluci, Tonini Fernanda, Schulte Rafael V, Demessiano Kely Z, Wolff Fellippe R, Delle-Monache Franco, Netz Daisy J A, Cechinel-Filho Valdir, de Freitas Rilton Alves, de Souza Márcia M, Meyre-Silva Christiane

机构信息

Centro de Ciências da Saúde, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brazil.

出版信息

J Pharm Pharmacol. 2014 Dec;66(12):1796-807. doi: 10.1111/jphp.12309. Epub 2014 Sep 12.

DOI:10.1111/jphp.12309

PMID:25212844

Abstract

OBJECTIVES

Litchi chinensis has been traditionally used in folk medicine to treat several ailments. In this study, we investigated the chemical composition, antioxidant and antinociceptive activity of L. chinensis leaves.

METHODS

The antioxidant capacity of the extract, fraction and compounds was evaluated using the 1,1-diphenyl-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and the liposome model with peroxyl radicals generated by 2,2'-azobis (2-amidinopropane) dihydrochloride radical. The pharmacological models of acute nociception used in mice were: writhing test with acetic acid (AA), hotplate (HP), glutamate (GLU), capsaicin (CP) and formalin (FM) tests.

KEY FINDINGS

The main compounds isolated were procyanidin A2 (PA2), procyanidin B2 (PB2) and (-)-epicatechin. The biochemical features of the crude extracts and their ethyl acetate fraction (EtOAcFR) presented high antioxidant activity, and the antioxidant activity of PA2 and PB2 was remarkably high, with DPPH and ABTS. The crude methanol extract (MeOHEXTR), EtOAcFR and PB2 were effective in reducing nociception in FM and HP models. MeOHEXTR and EtOAcFR treatments also reduced pain induced by GLU and AA. In the CP model, only EtOAcFR and PB2 were effective.

CONCLUSIONS

The results demonstrate the antinociceptive and antioxidant of MeOHEXTR, EtOAcFR and PB2.

摘要

目的

荔枝在民间医学中一直被用于治疗多种疾病。在本研究中，我们调查了荔枝叶的化学成分、抗氧化和抗伤害感受活性。

方法

使用1,1-二苯基-2-苦基肼（DPPH）和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法，以及由2,2'-偶氮双（2-脒基丙烷）二盐酸盐自由基产生过氧自由基的脂质体模型，评估提取物、馏分和化合物的抗氧化能力。在小鼠中使用的急性伤害感受药理学模型有：醋酸扭体试验（AA）、热板试验（HP）、谷氨酸试验（GLU）、辣椒素试验（CP）和福尔马林试验（FM）。

主要发现

分离得到的主要化合物是原花青素A2（PA2）、原花青素B2（PB2）和（-）-表儿茶素。粗提物及其乙酸乙酯馏分（EtOAcFR）的生化特征表现出高抗氧化活性，并且PA2和PB2的抗氧化活性在DPPH和ABTS测定中非常高。粗甲醇提取物（MeOHEXTR）、EtOAcFR和PB2在FM和HP模型中有效减轻伤害感受。MeOHEXTR和EtOAcFR处理也减轻了GLU和AA诱导的疼痛。在CP模型中，只有EtOAcFR和PB2有效。