Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, College of Medicine Campus, Idi-Araba, Lagos, Nigeria.
J Ethnopharmacol. 2012 May 7;141(1):282-9. doi: 10.1016/j.jep.2012.02.032. Epub 2012 Mar 1.
Cyathula prostrata (Linn) Blume (Amaranthaceae) is an annual herb widely used traditionally in the treatment of various inflammatory and pain related health disorders in Nigeria. The aim of this study is to evaluate the anti-inflammatory, analgesic and antioxidant activities of the methanolic extract of Cyathula prostrata (Linn) Blume.
The anti-inflammatory (phorbol 12-myristate 13-acetate (PMA)-induced reactive oxygen species (ROS), lipopolysaccharide (LPS) induced nitric oxide production in U937 macrophages, LPS-induced COX-2 expression, carrageenan-induced rat paw oedema, arachidonic acid-induced ear oedema and xylene-induced ear oedema), analgesic (acetic acid-induced writhing and hot plate tests) and antioxidant activities (DPPH [1,1-diphenyl-2-picrylhydrazyl] and lipid peroxidation assays) activities of the plant extract were investigated.
The methanolic extract of Cyathula prostrata did not show inhibitory activity in the in vitro PMA-induced reactive oxygen species, LPS-induced nitric oxide production and LPS-induced COX-2 expression assays. In the in vivo anti-inflammatory assays, the extract (50, 100 and 200mg/kg) showed a significant (P<0.05) dose-dependent inhibition in the carrageenan, arachidonic acid and xylene-induced tests. Cyathula prostrata produced a significant (P<0.05, 0.001) dose-dependent inhibition in the acetic acid and hot plate analgesic tests respectively. The plant extract did not exhibit any antioxidant activity in the DPPH and lipid peroxidation assays.
The results suggest that the methanolic extract of Cyathula prostrata possesses anti-inflammatory and analgesic activities and this authenticates the use of the plant in the traditional treatment of ailments associated with inflammation and pain.
地不容（Linn）Blume（苋科）是一种一年生草本植物，在尼日利亚传统上广泛用于治疗各种炎症和与疼痛相关的健康障碍。本研究旨在评估地不容（Linn）Blume 的甲醇提取物的抗炎、镇痛和抗氧化活性。
抗炎（佛波醇 12-肉豆蔻酸 13-醋酸酯（PMA）诱导的活性氧（ROS）、脂多糖（LPS）诱导 U937 巨噬细胞一氧化氮产生、LPS 诱导的 COX-2 表达、角叉菜胶诱导的大鼠足肿胀、花生四烯酸诱导的耳肿胀和二甲苯诱导的耳肿胀）、镇痛（醋酸诱导的扭体和热板试验）和抗氧化活性（DPPH [1,1-二苯基-2-苦基肼]和脂质过氧化测定）的植物提取物进行了研究。
地不容甲醇提取物在体外 PMA 诱导的活性氧、LPS 诱导的一氧化氮产生和 LPS 诱导的 COX-2 表达试验中没有显示出抑制活性。在体内抗炎试验中，提取物（50、100 和 200mg/kg）在角叉菜胶、花生四烯酸和二甲苯诱导的试验中表现出显著（P<0.05）剂量依赖性抑制。地不容在醋酸和热板镇痛试验中分别产生了显著（P<0.05，0.001）剂量依赖性抑制。植物提取物在 DPPH 和脂质过氧化测定中没有表现出任何抗氧化活性。
结果表明，地不容的甲醇提取物具有抗炎和镇痛活性，这证实了该植物在传统上用于治疗与炎症和疼痛相关的疾病。
