China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China; Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, PR China.
China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, PR China.
Food Chem. 2011 Aug 1;127(3):1342-7. doi: 10.1016/j.foodchem.2011.01.095. Epub 2011 Jan 28.
For quality control of Chinese materia medica (CMM), an attempt on fingerprint-efficacy study of artificial Calculus bovis was developed in this work. Chemical fingerprints of artificial C. bovis samples from ten different sources were determined by UPLC-ELSD and investigated by similarity analysis and hierarchical clustering analysis. Antibacterial effects of these samples on Escherichia coli growth were measured using microcalorimetry. The fingerprint-efficacy relationship of chemical fingerprint and antibacterial effect of artificial C. bovis were established by multi-linear regression analysis. Our results showed that the sources and places of production of artificial C. bovis had some important influence on the chemical fingerprints and antibacterial effects of this CMM. These artificial C. bovis could be grouped into four clusters according to their chemical fingerprints and antibacterial effects. Compounds cholic acid, taurocholate sodium, hyodeoxycholic acid and one unknown compound might be the major effective components for quality control of this CMM. Fingerprint-efficacy study provided a powerful way and some insight for the quality control of artificial C. bovis and other CCMs.
为了控制中药材的质量，本工作尝试对人工牛黄的指纹-药效关系进行研究。采用 UPLC-ELSD 法测定了来自 10 个不同来源的人工牛黄样品的化学指纹图谱，并通过相似度分析和层次聚类分析进行了考察。采用微量热法测量了这些样品对大肠杆菌生长的抑菌效果。通过多元线性回归分析，建立了人工牛黄化学指纹图谱与抑菌效果的关系。研究结果表明，人工牛黄的来源和产地对其化学指纹图谱和抑菌效果有一定的重要影响。根据其化学指纹图谱和抑菌效果，这些人工牛黄可以分为 4 个聚类。胆酸、牛磺胆酸钠、去氧胆酸和一个未知化合物可能是该中药材质量控制的主要有效成分。指纹-药效关系研究为人工牛黄和其他中药材的质量控制提供了一种强有力的方法和一些见解。
