骨髓间充质干细胞移植对烟雾吸入性损伤早期肺组织及血浆中血管内皮细胞生长因子的影响。
Effects of bone marrow-derived mesenchymal stem cells engraftment on vascular endothelial cell growth factor in lung tissue and plasma at early stage of smoke inhalation injury.
机构信息
Department of Critical Care Medicine (Zhu F), Department of Burns (Guo GH, Chen W, Wang NY), First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
出版信息
World J Emerg Med. 2010;1(3):224-8.
BACKGROUND
This study was undertaken to determine the effect of mesenchymal stem cells (MSCs) engraftment on vascular endothelial cell growth factor (VEGF) in lung tissue, plasma and extravascular lung water at early stage of smoke inhalation injury.
METHODS
A rabbit smoke inhalation injury model was established using a home-made smoke inhalation injury generator, and rabbits were divided into two groups randomly: a control group (S group, n=32) and a MSCs treatment group (M group, n=32). 10 ml PBS was injected via the ear marginal vein immediately at injury into the S group. Third generation MSCs with a concentration of 1×10(7)/10 ml PBS were injected via the ear marginal vein immediately at injury into the M group. VEGF in peripheral blood and lung tissue were measured at 0 (baseline), 2, 4 and 6 hours after injection respectively and analyzed. The right lungs of rabbits were taken to measure lung water mass fraction.
RESULTS
In the lung tissue, VEGF decreased gradually in the S group (P<0.05) and significantly decreased in the M group (P<0.05), but it increased more significantly than the values at the corresponding time points (P<0.05). In peripheral blood, VEGF increased gradually in the S group (P<0.05) and markedly increased in the M group (P<0.05), but it decreased more significantly than the values at corresponding time points (P<0.05).
CONCLUSION
MSCs engraftment to smoke inhalation injury could increase VEGF in lung tissue, decrease VEGF in plasma and reduce extravascular lung water, indicating its protective effect on smoke inhalation injury.
背景
本研究旨在探讨骨髓间充质干细胞（MSCs）移植对烟雾吸入性损伤早期肺组织、血浆和血管外肺水血管内皮生长因子（VEGF）的影响。
方法
采用自制烟雾吸入损伤发生器建立兔烟雾吸入性损伤模型，随机分为对照组（S 组，n=32）和 MSCs 治疗组（M 组，n=32）。S 组于损伤即刻经耳缘静脉注入 10 ml PBS；M 组于损伤即刻经耳缘静脉注入浓度为 1×10(7)/10 ml PBS 的第 3 代 MSCs。分别于注射后 0（基础）、2、4、6 小时取外周血和肺组织，检测 VEGF 并进行分析。取右肺组织测定肺水质量分数。
结果
肺组织中，S 组 VEGF 逐渐下降（P<0.05），M 组显著下降（P<0.05），但明显高于相应时间点（P<0.05）；S 组外周血 VEGF 逐渐升高（P<0.05），M 组明显升高（P<0.05），但明显低于相应时间点（P<0.05）。
结论
MSCs 移植对烟雾吸入性损伤可增加肺组织 VEGF，降低血浆 VEGF，减少血管外肺水，对烟雾吸入性损伤有保护作用。
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