Department of Emergency Medicine, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
World J Emerg Med. 2011;2(2):149-53. doi: 10.5847/wjem.j.1920-8642.2011.02.013.
Paraquat (PQ) intoxication causes lung oxidative stress damage. Saturated hydrogen saline, a newly explored antioxidant, has been documented to play a powerful antioxidant role in preventing oxidative stress damage. This study aimed to investigate the protective effects and the possible mechanisms of intoxication on rats with acute lung injury (ALI) caused by paraquat poisoning.
Thirty PQ poisoned rats were randomly divided into a PQ intoxication group (intoxication group), a saturated hydrogen saline intervention group (intervention group), and a control group, with 10 rats in each group. The first two groups accepted an intragastric administration of PQ at a dose of 50 mg/kg for every single rat, and the control group was fed with a same volume of normal saline. Five mL/kg of saturated hydrogen saline was given to the intervention group three times a day by peritoneal injection for three days after intoxication. Arterial blood gas was detected on the third day. The rats were executed and their lungs were taken for measurement of wet dry weight ratio, homogenate malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8-OhdG). Histological changes of the lungs were also observed.
Compared with the control group, the intoxication group had more serious hypoxemia, greater wet/dry weight ratio, higher MDA level, higher expression of 8-OhdG and more severe lung damage (P<0.01 or P<0.05). However, after intervention with saturated hydrogen saline, poisoned animals turned to have lighter hypoxemia, smaller wet/dry weight ratio, lower MDA level, lower expression of 8-OhdG, and milder lung damage (P<0.01 or P<0.05).
Saturated hydrogen saline is effective in preventing acute lung injury caused by PQ. Possibly, it can neutralize toxic oxygen radicals selectively and alleviate the oxidative stress injury induced by PQ.
百草枯（PQ）中毒会导致肺部氧化应激损伤。饱和氢生理盐水是一种新探索的抗氧化剂，已被证明在预防氧化应激损伤方面发挥强大的抗氧化作用。本研究旨在探讨饱和氢生理盐水对百草枯中毒大鼠急性肺损伤（ALI）的保护作用及其可能机制。
将 30 只百草枯中毒大鼠随机分为百草枯中毒组（中毒组）、饱和氢生理盐水干预组（干预组）和对照组，每组 10 只。前两组大鼠经灌胃给予 50mg/kg 百草枯，对照组给予等体积生理盐水。中毒后第 3 天，饱和氢生理盐水干预组每天腹腔注射 5ml/kg 饱和氢生理盐水 3 次。第 3 天检测动脉血气。处死大鼠，取肺组织测量湿干重比、匀浆丙二醛（MDA）和 8-羟基-2'-脱氧鸟苷（8-OhdG）。观察肺组织的病理变化。
与对照组相比，中毒组大鼠出现更严重的低氧血症，湿干重比更大，MDA 水平更高，8-OhdG 表达更高，肺损伤更严重（P<0.01 或 P<0.05）。然而，饱和氢生理盐水干预后，中毒动物的低氧血症减轻，湿干重比减小，MDA 水平降低，8-OhdG 表达减少，肺损伤减轻（P<0.01 或 P<0.05）。
饱和氢生理盐水对百草枯所致急性肺损伤有防治作用，其可能通过选择性中和毒性氧自由基减轻 PQ 诱导的氧化应激损伤。
