Bartolitius Lennart, Frickmann Hagen, Warnke Philipp, Ottl Peter, Podbielski Andreas
Eur J Microbiol Immunol (Bp). 2014 Sep;4(3):159-65. doi: 10.1556/EUJMI-D-14-00020. Epub 2014 Sep 11.
A nose model that allows for the comparison of different modes of sample acquisition as well as of nasal swab systems concerning their suitability to detect defined quantities of intranasal microorganisms, and further for training procedures of medical staff, was evaluated. Based on an imprint of a human nose, a model made of a silicone elastomer was formed. Autoclave stability was assessed. Using an inoculation suspension containing Staphylococcus aureus and Staphylococcus epidermidis, the model was compared with standardized glass plate inoculations. Effects of inoculation time, mode of sampling, and sample storage time were assessed. The model was stable to 20 autoclaving cycles. There were no differences regarding the optimum coverage from the nose and from glass plates. Optimum sampling time was 1 h after inoculation. Storage time after sampling was of minor relevance for the recovery. Rotating the swab around its own axis while circling the nasal cavity resulted in best sampling results. The suitability of the assessed nose model for the comparison of sampling strategies and systems was confirmed. Without disadvantages in comparison with sampling from standardized glass plates, the model allows for the assessment of a correct sampling technique due to its anatomically correct shape.
对一种鼻模型进行了评估，该模型可用于比较不同的样本采集模式以及鼻拭子系统在检测鼻腔内特定数量微生物方面的适用性，还可用于医护人员的培训程序。基于人类鼻子的印记，制作了一个由硅橡胶弹性体制成的模型。评估了其高压灭菌稳定性。使用含有金黄色葡萄球菌和表皮葡萄球菌的接种悬浮液，将该模型与标准化的玻璃板接种进行比较。评估了接种时间、采样模式和样本储存时间的影响。该模型在20次高压灭菌循环后仍保持稳定。从鼻子和玻璃板获得的最佳覆盖范围没有差异。最佳采样时间是接种后1小时。采样后的储存时间对回收率影响较小。在鼻腔内环绕时将拭子绕自身轴旋转可获得最佳采样结果。证实了所评估的鼻模型在比较采样策略和系统方面的适用性。与从标准化玻璃板采样相比没有劣势，该模型因其解剖学上正确的形状而能够评估正确的采样技术。
