Ross P W, Lough H
J Clin Pathol. 1978 May;31(5):430-3. doi: 10.1136/jcp.31.5.430.
Plain, buffered cotton-wool swabs and albumen-coated cotton-wool swabs were prepared with known numbers of the following: Haemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus, corynebacteria and Escherichia coli. Swabs were plated immediately (0 hours) after loading with a 0.1 ml standardised inoculum; each swab was plated out four times by streaking on both halves of two plates. At 0 hours recovery rates from first platings on solid media ranged from 3.3%-8.9%; from platings 1-4 ranges were 2.5%-5.7%. Both types of swabs gave similar results. The greater the delay in plating swabs at room temperature and 4 degrees C the lower was the recovery rate of H. influenzae and Strep. pneumoniae, though both species survived better on either kind of swab when these were held at 4 degrees C. There was a very small reduction in numbers of Staph. aureus from 24 to 48 hours but the recovery rate of corynebacteria was similar over the 48-hour period, at both room temperature and 4 degrees C. Twenty-four-hour counts of E. coli at room temperature were approximately five times greater than at 0 hours, and at 48 hours colonies were too numerous to count. At 4 degrees C, however, counts of E. coli fell during the 48-hour period.
使用已知数量的以下菌种制备普通、缓冲棉拭子和蛋白包被棉拭子：流感嗜血杆菌、肺炎链球菌、金黄色葡萄球菌、棒状杆菌和大肠杆菌。在加载0.1毫升标准化接种物后立即（0小时）将拭子接种平板；每个拭子通过在两个平板的两半上划线进行四次接种。在0小时时，固体培养基上首次接种的回收率在3.3%-8.9%之间；接种1-4次的回收率在2.5%-5.7%之间。两种拭子给出了相似的结果。在室温及4℃下接种拭子的延迟时间越长，流感嗜血杆菌和肺炎链球菌的回收率越低，不过当两种拭子保存在4℃时，这两种菌种在任何一种拭子上的存活情况都更好。从24小时到48小时，金黄色葡萄球菌的数量有非常小的减少，但在48小时期间，在室温及4℃下，棒状杆菌的回收率相似。在室温下，大肠杆菌24小时的菌落数大约是0小时时的五倍，在48小时时菌落多得无法计数。然而，在4℃下，大肠杆菌的菌落在48小时期间减少。
