Ma Juan, Wen Xiaosha, Mo Fengfeng, Wang Xiaoli, Shen Zhilei, Li Min
Department of Ship Hygiene, Second Military Medical University, NO.800 Xiangyin Road, Yangpu District, Shanghai, 200433, China.
Biol Trace Elem Res. 2014 Dec;162(1-3):242-51. doi: 10.1007/s12011-014-0115-4. Epub 2014 Sep 9.
Many controversies persist with respect to the dosage and therapeutic duration concerning iron deficiency anemia (IDA) treatment. To identify the most suitable cure, this study evaluated the effect of iron supplementation with different doses and for different time periods in rats with iron deficiency anemia. The rats were randomly divided into five groups [normal control (NC), low- iron diet control (LC), normal doses of iron group (NI), middle dose of iron group (MI), and high dose of iron group (HI)]. Each group was subdivided into two subgroups (2 and 4 weeks). The rats were maintained on low-iron diets and treated with oral iron dextran at different dosages. Finally, we investigated red blood cell parameters, iron absorption and metabolism, oxidative stress, and the antioxidant capacity. Our study indicated that through the administration of normal dose iron by gavage to IDA rats, the levels of the red blood cell parameters can be restored in only 2 weeks. In the HI group, iron absorption and transferrin receptor expressions were markedly reduced after 2 weeks. However, the iron content, ferritin and hepcidin expressions were notably increased, and the changes were more apparent after 4 weeks. With increasing doses of iron supplementation and durations of treatment, the liver malondialdehyde (MDA) content in the LC, MI, and HI groups was markedly increased, whereas the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were obviously reduced. This study demonstrated that the dose of iron treatment for IDA should be controlled in a safe range, and a reasonable duration is also critical for IDA therapeutics.
关于缺铁性贫血（IDA）治疗的剂量和治疗持续时间，仍存在许多争议。为了确定最合适的治疗方法，本研究评估了不同剂量和不同时间段补充铁剂对缺铁性贫血大鼠的影响。将大鼠随机分为五组[正常对照组（NC）、低铁饮食对照组（LC）、正常剂量铁组（NI）、中等剂量铁组（MI）和高剂量铁组（HI）]。每组再细分为两个亚组（2周和4周）。让大鼠维持低铁饮食，并给予不同剂量的口服右旋糖酐铁进行治疗。最后，我们研究了红细胞参数、铁吸收与代谢、氧化应激和抗氧化能力。我们的研究表明，通过对缺铁性贫血大鼠灌胃给予正常剂量的铁，仅2周就能使红细胞参数水平恢复。在高剂量铁组中，2周后铁吸收和转铁蛋白受体表达明显降低。然而，铁含量、铁蛋白和铁调素表达显著增加，4周后变化更为明显。随着铁补充剂量的增加和治疗时间的延长，低铁饮食对照组、中等剂量铁组和高剂量铁组肝脏丙二醛（MDA）含量明显增加，而超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性明显降低。本研究表明，缺铁性贫血的铁治疗剂量应控制在安全范围内，合理的治疗持续时间对缺铁性贫血治疗也至关重要。
