Johnson Robert D, Botch-Jones Sabra R, Flowers Tiffany, Lewis Connie A
Tarrant County Medical Examiner's Office, 200 Feliks Gwozdz Pl, Fort Worth, TX 76104, USA
FTox Consulting, 12 Green Street #1, Woburn, MA 01801, USA.
J Anal Toxicol. 2014 Oct;38(8):479-84. doi: 10.1093/jat/bku085.
As potent serotonin (5-HT2A) receptor agonists, the NBOMe class of drugs including 25B-, 25C-, 25D-, 25H-, 25I- and 25T2-NBOMe is frequently abused due to the intense hallucinations that they induce. From the limited literature available, the concentration of these NBOMe compounds reported in postmortem cases is exceedingly low. In most instances, published concentrations are <0.50 ng/mL. Therefore, the need for a sensitive, rapid and comprehensive analytical method for the quantification of these compounds was evident. In addition to the more publicized analog 25I-NBOMe, evaluation of 25B-, 25C-, 25D-, 25H and 25T2- in whole blood, plasma and urine was conducted. This publication presents the data obtained from the validation of a liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of these six NBOMe analogs. The method utilizes ultra-performance liquid chromatography technology for the separation followed by positive electrospray ionization of each analog. Limits of quantification for these analogs ranged from 0.01 to 0.02 ng/mL (10-20 pg/mL) with typical linear dynamic ranges of 0.01-20 ng/mL. Data for recovery, intraday control accuracy and precision, matrix effects, ion suppression/enhancement and analyte stability are included. Validation was completed in whole blood, plasma and urine. Short run times and high sensitivity afforded by this newly validated analytical method that allows for the detection of these six analogs in the most common toxicological matrices and can be applied to both ante- and postmortem specimens.
作为强效血清素（5-HT2A）受体激动剂，包括25B-NBOMe、25C-NBOMe、25D-NBOMe、25H-NBOMe、25I-NBOMe和25T2-NBOMe在内的NBOMe类药物因能引发强烈幻觉而常被滥用。从现有的有限文献来看，死后案例中报告的这些NBOMe化合物浓度极低。在大多数情况下，已公布的浓度<0.50 ng/mL。因此，显然需要一种灵敏、快速且全面的分析方法来定量这些化合物。除了更受关注的类似物25I-NBOMe外，还对全血、血浆和尿液中的25B-NBOMe、25C-NBOMe、25D-NBOMe、25H-NBOMe和25T2-NBOMe进行了评估。本出版物展示了通过液相色谱-串联质谱法同时定量这六种NBOMe类似物的验证所获得的数据。该方法采用超高效液相色谱技术进行分离，随后对每种类似物进行正电喷雾电离。这些类似物的定量限为0.01至0.02 ng/mL（10 - 20 pg/mL），典型线性动态范围为0.01 - 20 ng/mL。文中包含了回收率、日内对照准确度和精密度、基质效应、离子抑制/增强以及分析物稳定性的数据。验证在全血、血浆和尿液中完成。这种新验证的分析方法运行时间短且灵敏度高，能够在最常见的毒理学基质中检测到这六种类似物，并且可应用于生前和死后标本。
