Alshatwi Ali A, Hasan Tarique N, Alqahtani Ali M, Syed Naveed A, Shafi Gowhar, Al-Assaf Abdullah H, Al-Khalifa Abdulrahmann S
Molecular Cancer Biology Research Lab (MCBRL), Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Molecular Cancer Biology Research Lab (MCBRL), Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Environ Toxicol Pharmacol. 2014 Sep;38(2):653-62. doi: 10.1016/j.etap.2014.07.013. Epub 2014 Jul 29.
Catechins (flavan-3-ol) are a type of natural phenol and well-studied antioxidants. Catechin hydrate, also known as taxifolin; is non-mutagenic, low in toxicity compared to other immunomodulator antioxidants. We aimed to determine the potential of catechin hydrate to prevent the cyto-genotoxic effects of cadmium in lymphocytes; demonstrate the immuno-protective activity of catechin hydrate. Our previous study indicated that cadmium is apoptogenic. Lymphocytes were treated with catechin hydrate or cadmium and catechine hydrate combinations (range 0.1-100μM) to determine their effects on cell viability. Lymphocytes treated with 100μM catechin hydrate and 100μM cadmium showed cell viability 70.65±6.92% and 5.69±2.27%, respectively. In our previous study cadmium (10 and 20μM) induced apoptosis in 31.8% and 44.4% of lymphocytes, respectively. However, the percentage of apoptotic cells after treatment with the combination of cadmium and catechin hydrate was not significantly different from that of catechin hydrate (P>0.05). Only 7.3% and 10.5% of the lymphocytes were apoptotic after treatment with 10μM cadmium+10μM catechin hydrate and 20μM cadmium+20μM catechin hydrate, respectively. The anti-geno-cytotoxic and immuno-protective potential of catechin hydrate was also demonstrated by the non-significant expression of apoptosis-related genes after treatment with catechin hydrate.
儿茶素（黄烷-3-醇）是一类天然酚类物质，也是经过充分研究的抗氧化剂。儿茶素水合物，也被称为紫杉叶素；它无致突变性，与其他免疫调节抗氧化剂相比毒性较低。我们旨在确定儿茶素水合物预防镉对淋巴细胞产生细胞遗传毒性作用的潜力；证明儿茶素水合物的免疫保护活性。我们之前的研究表明镉具有诱导细胞凋亡的作用。用儿茶素水合物或镉以及儿茶素水合物组合（浓度范围为0.1 - 100μM）处理淋巴细胞，以确定它们对细胞活力的影响。用100μM儿茶素水合物和100μM镉处理的淋巴细胞的细胞活力分别为70.65±6.92%和5.69±2.27%。在我们之前的研究中，镉（10μM和20μM）分别诱导31.8%和44.4%的淋巴细胞凋亡。然而，镉与儿茶素水合物联合处理后凋亡细胞的百分比与儿茶素水合物处理后的相比无显著差异（P>0.05）。用10μM镉 + 10μM儿茶素水合物和20μM镉 + 20μM儿茶素水合物处理后，分别只有7.3%和10.5%的淋巴细胞发生凋亡。儿茶素水合物处理后凋亡相关基因的表达无显著变化，这也证明了儿茶素水合物的抗基因细胞毒性和免疫保护潜力。
