Milošević-Djordjević Olivera, Radović Jakovljević Marina, Marković Aleksandra, Stanković Milan, Ćirić Andrija, Marinković Dragoslav, Grujičić Darko
Department of Biology and Ecology, Faculty of Science, University of Kragujevac , Kragujevac , Serbia.
Department of Genetics, Faculty of Medical Sciences, University of Kragujevac , Kragujevac , Serbia.
Turk J Biol. 2018 Apr 27;42(2):152-162. doi: 10.3906/biy-1707-36. eCollection 2018.
Teucrium species have been used in traditional medicine for treatment of different diseases. The aim of this study was to investigate polyphenolic contents by high-performance liquid chromatography (HPLC), and the genotoxic effect of methanolic extracts of Teucrium polium and Teucrium scordium using the cytokinesis-block micronucleus (CBMN) assay on human peripheral blood lymphocytes (PBLs) from healthy donors. The HPLC analysis showed that extracts consist of phenolic acid (gallic, vanillic, caefic, chlorogenic, p-coumaric, sinapic) and flavonoids (catechin, rutin, myricetin, luteolin, quercetin and apigenin). Cultures were treated with extracts of both plants separately and in combinations with mitomycin C (MMC). In separate treatments, both herbal extracts significantly induced micronucleus (MN) frequency only at the highest concentrations. All concentrations of T. scordium , except the lowest, and all concentrations of T. polium extracts in combined treatment with MMC significantly reduced the frequency of MN. The extract of T. polium did not significantly aefct the nuclear division index (NDI), whereas T. scordium in higher concentrations, separately and in combined treatment with MMC, significantly decreased the NDI value. Our results suggest that both herbal extracts in combination with MMC have antimutagenic (T. polium) and proapoptotic effects (T. scordium), which indicates their protective effects in PBLs.
香科科属植物已被用于传统医学治疗各种疾病。本研究的目的是通过高效液相色谱法（HPLC）研究多酚含量，并使用胞质分裂阻滞微核（CBMN）试验，检测香科科和蒜味香科科甲醇提取物对健康供体人外周血淋巴细胞（PBLs）的遗传毒性作用。HPLC分析表明，提取物含有酚酸（没食子酸、香草酸、咖啡酸、绿原酸、对香豆酸、芥子酸）和黄酮类化合物（儿茶素、芦丁、杨梅素、木犀草素、槲皮素和芹菜素）。将两种植物的提取物分别以及与丝裂霉素C（MMC）联合处理细胞培养物。在单独处理中，两种草药提取物仅在最高浓度时显著诱导微核（MN）频率。在与MMC联合处理时，除最低浓度外，蒜味香科科的所有浓度以及香科科提取物的所有浓度均显著降低了MN频率。香科科提取物对核分裂指数（NDI）没有显著影响，而蒜味香科科在较高浓度下，单独处理以及与MMC联合处理时，均显著降低了NDI值。我们的结果表明，两种草药提取物与MMC联合使用具有抗诱变作用（香科科）和促凋亡作用（蒜味香科科），这表明它们对PBLs具有保护作用。
