用于福尔马林固定石蜡包埋组织的多重miRNA荧光原位杂交
Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues.
作者信息
Renwick Neil, Cekan Pavol, Bognanni Claudia, Tuschl Thomas
机构信息
Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY, 10065, USA.
出版信息
Methods Mol Biol. 2014;1211:171-87. doi: 10.1007/978-1-4939-1459-3_14.
Abstract
Multiplexed miRNA fluorescence in situ hybridization (miRNA FISH) is an advanced method for visualizing differentially expressed miRNAs, together with other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss by diffusion, probe mishybridization, and signal detection and signal amplification issues. Here, we describe a reliable and adjustable method for visualizing and normalizing miRNA signals in formalin-fixed paraffin-embedded (FFPE) tissue sections.
摘要
多重miRNA荧光原位杂交（miRNA FISH）是一种用于在存档组织中可视化差异表达的miRNA以及其他参考RNA的先进方法。一些miRNA由于其丰度和细胞类型特异性，是优秀的疾病生物标志物。然而，由于扩散导致的损失、探针错配杂交以及信号检测和信号放大问题，这些短RNA分子难以可视化。在这里，我们描述了一种在福尔马林固定石蜡包埋（FFPE）组织切片中可视化和标准化miRNA信号的可靠且可调节的方法。
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