Hung Chi-Jr, Hsu Hsin-I, Lin Chi-Chang, Huang Tsui-Hsien, Wu Buor-Chang, Kao Chia-Tze, Shie Ming-You
School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan.
Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan.
J Endod. 2014 Nov;40(11):1802-9. doi: 10.1016/j.joen.2014.07.016. Epub 2014 Sep 11.
It has been proved that integrin αv activity is related to cell proliferation, differentiation, migration, and organ development. However, the biological functions of integrin αv in human dental pulp cells (hDPCs) cultured on silicate-based materials have not been explored. The aim of this study was to investigate the role of integrin αv in the proliferation and odontogenic differentiation of hDPCs cultured with the effect of calcium silicate (CS) cement and β-tricalcium phosphate (TCP) cement.
In this study, hDPCs were cultured on CS and TCP materials, and we evaluated fibronectin (FN) secretion and integrin αv expression during the cell attachment stage. After small interfering RNA transfection targeting integrin αv, the proliferation and odontogenesis differentiation behavior of hDPCs were analyzed.
The results indicate that CS releases Si ion-increased FN secretion and adsorption, which promote cell attachment more effectively than TCP. The CS cement facilitates FN and αv subintegrin expression. However, the FN adsorption and integrin expression of TCP are similar to that observed in the control dish. Integrin αv small interfering RNA inhibited odontogenic differentiation of hDPCs with the decreased formation of mineralized nodules on CS. It also down-regulated the protein expression of multiple markers of odontogenesis and the expression of dentin sialophosphoprotein protein.
These results establish composition-dependent differences in integrin binding and its effectiveness as a mechanism regulating cellular responses to biomaterial surface.
已证实整合素αv的活性与细胞增殖、分化、迁移及器官发育有关。然而，整合素αv在基于硅酸盐的材料上培养的人牙髓细胞（hDPCs）中的生物学功能尚未得到探索。本研究的目的是研究整合素αv在硅酸钙（CS）水泥和β-磷酸三钙（TCP）水泥作用下培养的hDPCs增殖和牙源性分化中的作用。
在本研究中，hDPCs在CS和TCP材料上培养，我们评估了细胞附着阶段的纤连蛋白（FN）分泌和整合素αv表达。在靶向整合素αv的小干扰RNA转染后，分析了hDPCs的增殖和牙源性分化行为。
结果表明，CS释放的硅离子增加了FN的分泌和吸附，比TCP更有效地促进细胞附着。CS水泥促进FN和αv亚整合素表达。然而，TCP的FN吸附和整合素表达与对照培养皿中观察到的相似。整合素αv小干扰RNA抑制了hDPCs的牙源性分化，CS上矿化结节的形成减少。它还下调了多种牙源性标志物的蛋白表达和牙本质涎磷蛋白的表达。
这些结果表明，整合素结合及其作为调节细胞对生物材料表面反应机制的有效性存在成分依赖性差异。
