Department of Endodontics, Tamil Nadu Government Dental College, Chennai, India.
J Endod. 2013 Jun;39(6):806-12. doi: 10.1016/j.joen.2012.12.036. Epub 2013 Feb 13.
The aim of this study was to investigate the combined effect of statin and α-tricalcium phosphate (α-TCP) on odontoblastic differentiation of human dental pulp cells and to compare them with mineral trioxide aggregate (MTA).
Experimental cements were prepared with TCP containing simvastatin and atorvastatin. Cell proliferation, cell adherence on a dentin disc, alkaline phosphatase (ALP) activity, expression of osteogenic/odontoblastic markers, and mineralization of the human dental pulp cells on experimental cement and MTA were assessed.
The cell growth and ALP activity of TCP containing simvastatin-treated cells was greater than MTA-treated cells. The mineralization and messenger RNA expression of markers (ie, dentin sialophosphoprotein, dentin matrix protein 1, bone morphogenetic protein 2, ALP, and osteonectin) of TCP containing simvastatin- and TCP containing atorvastatin-treated cells were comparable with MTA-treated cells. The enhanced cell proliferation and similar level of ALP of TCP-treated cells compared with the control indicate that α-TCP is an effective osteoconductive material. The differentiation effect observed in TCP containing simvastatin- and TCP containing atorvastatin-treated cells is attributed to the effect of statin.
The results suggest that α-TCP can be used for local delivery of statin as a pulp capping material to accelerate reparative dentin formation.
本研究旨在探讨他汀类药物和 α-磷酸三钙（α-TCP）联合对人牙髓细胞成牙本质分化的影响，并将其与矿化三氧化物凝聚体（MTA）进行比较。
用含有辛伐他汀和阿托伐他汀的 TCP 制备实验性水门汀。评估人牙髓细胞在实验性水门汀和 MTA 上的细胞增殖、牙本质盘上的细胞黏附、碱性磷酸酶（ALP）活性、成骨/成牙本质标志物的表达和矿化。
含辛伐他汀处理细胞的 TCP 细胞生长和 ALP 活性大于 MTA 处理细胞。含辛伐他汀和含阿托伐他汀的 TCP 处理细胞的矿化和标志物（即牙本质涎磷蛋白、牙本质基质蛋白 1、骨形态发生蛋白 2、ALP 和骨粘连蛋白）的信使 RNA 表达与 MTA 处理细胞相当。与对照组相比，TCP 处理细胞的细胞增殖增强和 ALP 水平相似表明 α-TCP 是一种有效的骨传导材料。含辛伐他汀和含阿托伐他汀的 TCP 处理细胞中观察到的分化效果归因于他汀类药物的作用。
结果表明，α-TCP 可用作局部递送他汀类药物的牙髓覆盖材料，以加速修复性牙本质形成。
