College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu 241000, PR China.
College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu 241000, PR China.
Biosens Bioelectron. 2015 Feb 15;64:212-8. doi: 10.1016/j.bios.2014.08.083. Epub 2014 Sep 4.
A molecular imprinting polymer (MIP) based electrochemical sensor was successfully prepared for dopamine (DA) recognition and detection using pyrrole-phenylboronic acid (py-PBA) as a novel electropolymerized monomer. py-PBA could form cyclic boronic ester bond with DA, thus endowing a double recognition capacity of the sensor to DA in the combination of the imprinted effect of MIP. Compared with the sensor prepared using pyrrole or phenylboronic acid as electropolymerized monomer, the present sensor exhibited a remarkable high imprinted factor to DA. The influence factors including pH value, the mole ratio between monomer and template molecule, electropolymerization scan rate, and scan cycles of electropolymerization process were investigated and optimized. Under the optimal conditions, the sensor could recognize DA from its analogs and monosaccharides. A linear ranging from 5.0 × 10(-8) to 1.0 × 10(-5) mol/L for the detection of DA was obtained with a detection limit of 3.3 × 10(-8) mol/L (S/N = 3). The sensor has been applied to analyze DA in injection samples with satisfactory results.
一种基于分子印迹聚合物（MIP）的电化学传感器，成功地使用吡咯-苯硼酸（py-PBA）作为新型的电聚合单体用于多巴胺（DA）的识别和检测。py-PBA 可以与 DA 形成环状硼酸酯键，从而赋予传感器对 DA 的双重识别能力，结合 MIP 的印迹效应。与使用吡咯或苯硼酸作为电聚合单体制备的传感器相比，本传感器对 DA 表现出显著的高印迹因子。研究并优化了包括 pH 值、单体与模板分子摩尔比、电聚合扫描速率和电聚合循环次数在内的影响因素。在最佳条件下，该传感器能够从其类似物和单糖中识别 DA。DA 的检测范围为 5.0×10(-8)至 1.0×10(-5)mol/L，检测限为 3.3×10(-8)mol/L（S/N=3）。该传感器已应用于注射液中 DA 的分析，结果令人满意。
