Horcajada M-N, Sanchez C, Membrez Scalfo F, Drion P, Comblain F, Taralla S, Donneau A-F, Offord E A, Henrotin Y
Nestle Research Center, Nutrition and Health Research, Vers-Chez-les-Blanc, Lausanne 26 1000, Switzerland.
Bone and Cartilage Research Unit, Arthropôle, University of Liège, Institute of Pathology, CHU Sart-Tilman, 4000 Liège, Belgium.
Osteoarthritis Cartilage. 2015 Jan;23(1):94-102. doi: 10.1016/j.joca.2014.08.016. Epub 2014 Sep 8.
To assess the potential protective effects of three polyphenols oleuropein, rutin and curcumin, on joint ageing and osteoarthritis (OA) development.
Sixty 4-week-old Dunkin-Hartley guinea pigs were randomized into four groups and received daily during 31 weeks either standard guinea pig diet (control group) or a standard guinea pig diet enriched with oleuropein (0.025%), rutin (0.5%) or rutin/curcumin (0.5%/0.25%) association. Biomarkers of OA (Coll2-1, Coll2-1NO2, Fib3-1, Fib3-2, ARGS), as well as inflammation prostaglandin E2 (PGE2) were quantified in the serum. Histological assessments of knee cartilage and synovial membrane were performed at week 4 (five young reference guinea pigs) and week 35.
At week 35, guinea pigs in the control group spontaneously developed significant cartilage lesions with mild synovial inflammation. The histological scores of cartilage lesions and synovitis were well correlated with the increased level of serum biomarkers. Histologically, all treatments significantly reduced the cartilage degradation score (P < 0.01), but only oleuropein significantly decreased the synovial histological score (P < 0.05) and serum PGE2 levels (P < 0.01) compared to the control group. Coll2-1 was decreased by rutin and the combination of rutin/curcumin, Fib3-1 and Fib3-2 were only decreased by the rutin/curcumin mixture, while Coll2-1NO2 was significantly decreased by all treatments (P < 0.05).
Oleuropein and rutin ± curcumin significantly slowed down the progression of spontaneous OA lesions in guinea pigs. While no additive effect was seen in the curcumin + rutin group, the differential effects of oleuropein and rutin on inflammatory and cartilage catabolic markers suggest an interesting combination for future studies in OA protection.
评估橄榄苦苷、芦丁和姜黄素这三种多酚对关节衰老和骨关节炎（OA）发展的潜在保护作用。
将60只4周龄的邓金-哈特利豚鼠随机分为四组，在31周内每天分别给予标准豚鼠饮食（对照组）或富含橄榄苦苷（0.025%）、芦丁（0.5%）或芦丁/姜黄素（0.5%/0.25%）组合的标准豚鼠饮食。对血清中OA生物标志物（Coll2-1、Coll2-1NO2、Fib3-1、Fib3-2、ARGS）以及炎症介质前列腺素E2（PGE2）进行定量分析。在第4周（五只年轻的对照豚鼠）和第35周对膝关节软骨和滑膜进行组织学评估。
在第35周时，对照组豚鼠自发出现明显的软骨损伤并伴有轻度滑膜炎。软骨损伤和滑膜炎的组织学评分与血清生物标志物水平的升高密切相关。组织学结果显示，与对照组相比，所有处理均显著降低了软骨降解评分（P < 0.01），但只有橄榄苦苷显著降低了滑膜组织学评分（P < 0.05）和血清PGE2水平（P < 0.01）。芦丁以及芦丁/姜黄素组合降低了Coll2-1水平，只有芦丁/姜黄素混合物降低了Fib3-1和Fib3-2水平，而所有处理均显著降低了Coll2-1NO2水平（P < 0.05）。
橄榄苦苷和芦丁±姜黄素显著减缓了豚鼠自发性OA病变的进展。虽然姜黄素+芦丁组未观察到相加效应，但橄榄苦苷和芦丁对炎症和软骨分解代谢标志物的不同作用表明，它们是未来OA保护研究中一个有趣的组合。
